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Binding affinity to human RANKL assessed as dissociation constant by surface plasmon resonance method
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to GST-fused human RANKL extracellular domain (Lys159 to Asp317 residues) expressed in Escherichia coli BL21(DE3) pLysS by fluorescence spectrophotometric assay
Assay data:13 Active, 40 Tested
Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in RANKL/M-CSF-induced TRAP activity preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days
Assay data:11 Active, 1 Activity ≤ 1 µM, 11 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human soluble RANKL trimerization assessed as increase in RANKL monomer level at 1:3 to 1:10 ratio of DSS to RANKL preincubated for 1 hr followed by DSS addition and measured after 1 hr by SDS-PAGE analysis
Potency index, ratio of SPD-304 Kd to test compound Kd for binding affinity to GST-fused human RANKL extracellular domain (Lys159 to Asp317 residues) expressed in Escherichia coli BL21(DE3) pLysS by fluorescence spectrophotometric assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in RANKL/M-CSF-induced TRAP activity at 5 uM preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days
Assay data:8 Active, 37 Tested
Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in RANKL/M-CSF-induced TRAP activity at 5 to 10 uM preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days
Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in phalloidin-labeled actin ring formation at 5 to 10 uM preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days by Alexa Fluor 488-conjugated phalloidin/DAPI-staining based fluorescence microscopic analysis
Inhibition of human soluble RANKL trimerization assessed as increase in RANKL monomer level preincubated for 1 hr followed by DSS addition and measured after 1 hr by SDS-PAGE analysis
Assay data:1 Tested
Inhibition of human RANKL-induced osteoclastogenesis in RANKL cultured mouse bone marrow cells assessed as reduction in phalloidin-labeled actin ring formation preincubated with RANKL for 1 hr followed by cell addition and subsequent media replenishment every 48 hrs for 4 days by Alexa Fluor 488-conjugated phalloidin/DAPI-staining based fluorescence microscopic analysis
Inhibition of human soluble RANKL trimerization assessed as increase in RANKL monomer level at 1:3 ratio of DSS to RANKL preincubated for 1 hr followed by DSS addition and measured after 1 hr by SDS-PAGE analysis
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated NFATc1 expression by measuring NFATc1 protein expression at 2 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis relative to positive control (Rvb = 1 No_unit)
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated NFATc1 expression by measuring NFATc1 protein expression at 5 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis relative to positive control (Rvb = 1 No_unit)
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated NFATc1 expression by measuring NFATc1 protein expression at 10 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis relative to positive control (Rvb = 1 No_unit)
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated cFOS expression by measuring cFOS protein expression at 2 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis relative to positive control (Rvb = 1 No_unit)
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated cFOS expression by measuring cFOS protein expression at 5 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis relative to positive control (Rvb = 1 No_unit)
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated cFOS expression by measuring cFOS protein expression at 10 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis relative to positive control (Rvb = 1 No_unit)
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated cFOS expression at 2 to 10 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis
Inhibition of RANKL (unknown origin) in RANKL cultured mouse RAW264.7 cells assessed as reduction in RANKL/RANK-interaction mediated NFATc1 expression at 2 to 10 uM preincubated for 1 hr followed by cell culture addition and measured after 2 days by Western blot analysis
Binding affinity to human RANKL measured after 1 hr by fluorescence assay
Assay data:2 Active, 3 Tested
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