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Inhibition of human recombinant PNMT using AdoMet and DL-norepinephrine hydrocloride as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by fluorescence based analysis relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Radiochemical Assay of PNMT Inhibitors from Article 10.1021/jm060466d: "Comparison of the binding of 3-fluoromethyl-7-sulfonyl-1,2,3,4-tetrahydroisoquinolines with their isosteric sulfonamides to the active site of phenylethanolamine N-methyltransferase."
Assay data:21 Active, 10 Activity ≤ 1 µM, 27 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Primary Screen for Target Class Profiling of Small Molecule Inhibitors of Methyltransferases
Assay data:1471 Active, 24661 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Confirmatory Screen for Target Class Profiling of Small Molecule Inhibitors of Methyltransferases
Assay data:902 Active, 1111 Tested
Non-competitive inhibition of C-terminal hexahistidine tag in human recombinant PNMT expressed in Escherichia coli assessed as intercept inhibition constant in presence of 5 uM PEA as substrate and AdoMet as co-substrate by Sigma-plot analysis
Assay data:14 Active, 13 Activity ≤ 1 µM, 21 Tested
Non-competitive inhibition of C-terminal hexahistidine tag in human recombinant PNMT expressed in Escherichia coli assessed as slope inhibition constant in presence of 5 uM PEA as substrate and AdoMet as co-substrate by Sigma-plot analysis
Assay data:17 Active, 14 Activity ≤ 1 µM, 21 Tested
Competitive inhibition of C-terminal hexahistidine tag in human recombinant PNMT expressed in Escherichia coli assessed as inhibition constant using PEA as substrate in presence of 100 uM AdoMet as co-substrate by Sigma-plot analysis
Assay data:16 Active, 14 Activity ≤ 1 µM, 21 Tested
Inhibition of human PNMT expressed in Escherichia coli at 1 uM assessed as reduction in SAH level using DL-normetanephrine as substrate in presence of SAM incubated for 45 mins by LC-MS/MS analysis relative to control
Assay data:1 Tested
Inhibition of human PNMT expressed in Escherichia coli at 10 uM assessed as reduction in SAH level using DL-normetanephrine as substrate in presence of SAM incubated for 45 mins by LC-MS/MS analysis relative to control
Inhibition of human N-terminal His6 tagged PNMT using norepinephrine as substrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay
Inhibition of human N-terminal His6 tagged PNMT assessed as enzyme activity at 100 uM using norepinephrine as substrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control
Inhibition of human N-terminal His6 tagged PNMT assessed as enzyme activity at 33 uM using norepinephrine as substrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control
Inhibition of human N-terminal His6 tagged PNMT assessed as enzyme activity at 11 uM using norepinephrine as substrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control
Inhibition of human N-terminal His6 tagged PNMT assessed as enzyme activity at 3.7 uM using norepinephrine as substrate preincubated for 10 mins in presence of AdoMet followed by substrate addition by fluorescence based SAHH enzyme coupled assay relative to control
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Human Phenylethanolamine N-methyltransferase (Catecholamine turnover)
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
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