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Inhibition of Thermus thermophilus HB8 GCYH1A expressed in Escherichia coli BL21 (DE3) using GTP as substrate incubated for 60 to 180 mins by spectrofluorimetric analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 15 mins in presence of SAM and substrate followed by Ti(3+)citrate addition and measured by discontinuous HPLC analysis
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 IleRS H581N/L583F double mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 IleRS H581S/L583I double mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 IleRS H581L/L583H double mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Inhibition of recombinant Thermus thermophilus HB8 IleRS L583F mutant assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of recombinant Thermus thermophilus HB8 wild type IleRS assessed as suppression of L-[14C]isoleucyl-tRNA(Ile) formation
Activity of Thermus thermophilus HB8 MetRS by ATP:PPi exchange assay in presence of methionine
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Thermus thermophilus HB8 IPMDH assessed as formation of NADH by Lineweaver-Burke plot analysis
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Reversible inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster assessed as restoration of enzyme activity at 2 mM using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 1 hr in presence of SAM, substrate and Ti(3+)citrate followed by compound washout and subsequent addition of SAM, substrate and Ti(3+)citrate measured after 1 hr by HPLC analysis
Substrate activity at N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster assessed as enzyme-mediated 3-(4-((2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)-2-carboxybutyl)benzoic acid formation
Activity of Thermus thermophilus HB8 MetRS by ATP:PPi exchange assay in presence of ATP
Experimentally measured binding affinity data (Kd) for protein-ligand complexes derived from PDB
Assay data:3916 Active, 206 Activity ≤ 1 nM, 1830 Activity ≤ 1 µM, 3916 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Experimentally measured binding affinity data derived from PDB
Assay data:3073 Active, 3073 Tested
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