HGNC Approved Gene Symbol: OOSP2
Cytogenetic location: 11q12.1 Genomic coordinates (GRCh38): 11:60,040,409-60,048,044 (from NCBI)
OOSP2 is an oocyte-secreted protein involved in oocyte maturation (Hu et al., 2022).
By in silico analysis, Paillisson et al. (2005) identified Oosp2 as an oocyte-specific gene in mouse. Oosp2 protein contains a signal peptide and a partial zona pellucida-like domain.
Using bioinformatic and RT-PCR analyses, Abbasi et al. (2020) confirmed that Oosp2 was an oocyte-enriched gene in mouse. OOSP2 is conserved in a variety of mammalian species, including human, and OOSP proteins share a significant degree of homology.
By transcriptome and translatome sequencing, Hu et al. (2022) identified OOSP2 as a differentially expressed gene during human oocyte maturation. Mouse Oosp2 has a polyadenylation signal (PAS) in its 3-prime UTR, whereas human OOSP2 has a PAS and a cytoplasmic polyadenylation element (CPE) in its 3-prime UTR. OOSP2 was highly expressed in human oocytes, and the authors found that CPEB4 (610607) bound to the 3-prime UTR of OOSP2 and upregulated its translation.
Gross (2023) mapped the OOSP2 gene to chromosome 11q12.1 based on an alignment of the OOSP2 sequence (GenBank BC036256) with the genomic sequence (GRCh38).
Paillisson et al. (2005) mapped the Oosp2 gene to mouse chromosome 19A.
Hu et al. (2022) found that treatment of germinal vesicle (GV)-stage human oocytes with purified recombinant OOSP2 increased the percentage of mature oocytes. OOSP2 treatment induced expression of many genes in oocytes, mainly by upregulating their translation.
Abbasi et al. (2020) found that Oosp2 -/- mice were viable with no overt abnormalities. Oosp2 -/- females were fertile when mated with wildtype males. Triple-knockout mice lacking Oosp1, Oosp2, and Oosp3 were also fertile, indicating that fertility of Oosp2 -/- females was not due to compensation by other Oosp family genes. However, the litter sizes of triple-knockout females were reduced, likely due to reduction in viable embryos.
Abbasi, F., Kodani, M., Emori, C., Kiyozumi, D., Mori, M., Fujihara, Y., Ikawa, M. CRISPR/Cas9-mediated genome editing reveals Oosp family genes are dispensable for female fertility in mice. Cells 9: 821, 2020. [PubMed: 32231122] [Full Text: https://doi.org/10.3390/cells9040821]
Gross, M. B. Personal Communication. Baltimore, Md. 2/23/2023.
Hu, W., Zeng, H., Shi, Y., Zhou, C., Huang, J., Jia, L., Xu, S., Feng, X., Zeng, Y., Xiong, T., Huang, W., Sun, P., and 13 others. Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation. Nature Commun. 13: 5114, 2022. [PubMed: 36042231] [Full Text: https://doi.org/10.1038/s41467-022-32791-2]
Paillisson, A., Dade, S., Callebaut, I., Bontoux, M., Dalbies-Tran, R., Vaiman, D., Monget, P. Identification, characterization and metagenome analysis of oocyte-specific genes organized in clusters in the mouse genome. BMC Genomics 6: 76, 2005. [PubMed: 15907208] [Full Text: https://doi.org/10.1186/1471-2164-6-76]