Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF606
Cytogenetic location: 19q13.43 Genomic coordinates (GRCh38): 19:57,977,053-58,003,346 (from NCBI)
ZNF606 belongs to a large family of transcription factors characterized by an N-terminal Kruppel-associated box (KRAB) domain and tandem C-terminal C2H2-type zinc fingers (Ou et al., 2005).
By sequencing clones obtained from a size-fractionated fetal brain cDNA library, Nagase et al. (2001) cloned ZNF606, which they designated KIAA1852. The deduced 948-amino acid protein shares significant similarity with ZNF184 (602277). RT-PCR ELISA detected moderate to high ZNF606 expression in all adult and fetal tissues and all specific adult brain regions examined. Highest expression was detected in cerebellum.
Using degenerate primers based on the KRAB domain sequence to screen a 20-week-old embryonic human heart cDNA library, followed by EST database analysis and 5-prime and 3-prime RACE, Ou et al. (2005) cloned ZNF606, which they called ZNF328. The deduced 792-amino acid protein has a calculated molecular mass of 88 kD. It has an N-terminal KRAB domain and 15 tandem Kruppel C2H2-type zinc fingers in its C-terminal half. ZNF606 shares highest similarity with ZNF234 (604750). Northern blot analysis revealed variable expression of a 4.5-kb transcript in all adult and fetal tissues examined, with highest expression in adult heart, brain, liver, muscle, and pancreas, and in fetal heart, cerebrum, cerebellum, liver, muscle, and pancreas. Fluorescence-tagged ZNF606 localized to nuclei of transfected COS-7 cells.
By cotransfecting COS-7 cells with ZNF606 and a reporter gene system, Ou et al. (2005) showed that ZNF606 repressed transcription when targeted to template DNA. ZNF606 specifically reduced the transcriptional activities of the serum response element (SRE) and AP1 (165160). Deletion analysis showed that the KRAB domain of ZNF606 functioned as a basal repression domain.
Ou et al. (2005) determined that the ZNF606 gene contains 8 exons and spans approximately 28 kb.
Using radiation hybrid analysis, Nagase et al. (2001) mapped the ZNF606 gene to chromosome 19. Ou et al. (2005) mapped the ZNF606 gene to chromosome 19q13.4 by genomic sequence analysis.
Nagase, T., Nakayama, M., Nakajima, D., Kikuno, R., Ohara, O. Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 8: 85-95, 2001. [PubMed: 11347906] [Full Text: https://doi.org/10.1093/dnares/8.2.85]
Ou, Y., Wang, S., Cai, Z., Wang, Y., Wang, C., Li, Y., Li, F., Yuan, W., Liu, B., Wu, X., Liu, M. ZNF328, a novel human zinc-finger protein, suppresses transcriptional activities of SRE and AP-1. Biochem. Biophys. Res. Commun. 333: 1034-1044, 2005. [PubMed: 15964554] [Full Text: https://doi.org/10.1016/j.bbrc.2005.05.192]