Entry - *613431 - HAUS AUGMIN-LIKE COMPLEX, SUBUNIT 4; HAUS4 - OMIM

 
 
* 613431

HAUS AUGMIN-LIKE COMPLEX, SUBUNIT 4; HAUS4


Alternative titles; symbols

CHROMOSOME 14 OPEN READING FRAME 94; C14ORF94


HGNC Approved Gene Symbol: HAUS4

Cytogenetic location: 14q11.2     Genomic coordinates (GRCh38): 14:22,946,228-22,957,090 (from NCBI)


TEXT

▼ Description

HAUS4 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008; Uehara et al., 2009).


▼ Cloning and Expression

Using mass spectrometry to identify proteins immunoprecipitated with the augmin complex subunit DGT6 (HAUS6; 613433) from HeLa cell mitotic extracts, Uehara et al. (2009) identified HAUS4, which they called C14ORF94. The deduced 363-amino acid protein has a calculated molecular mass of 42.4 kD.


▼ Gene Function

Uehara et al. (2009) found that epitope-tagged HAUS4 immunoprecipitated endogenous DGT6 from HeLa cells. Like other augmin subunits, fluorescence-tagged HAUS4 localized to the metaphase spindle and accumulated at centrosomes during interphase. Knockdown of HAUS4 via RNA interference reduced the signals for gamma-tubulin (TUBG1; 191135) and microtubules at mitotic spindles.

Using RNA interference, Lawo et al. (2009) independently showed that HAUS4 is a critical component of the human augmin complex, and they confirmed interaction of HAUS4 with the other 7 augmin subunits by coimmunoprecipitation analysis. Knockdown of HAUS4 or any of the other augmin subunits resulted in destabilization of kinetochore microtubules, formation of multipolar spindles, fragmentation of centrosomes, and increased centrosome size. These severe mitotic defects were alleviated by codepletion of the nuclear mitotic apparatus (NUMA; see 164009), suggesting that augmin and NUMA regulate opposing activities.


▼ Mapping

Hartz (2010) mapped the HAUS4 gene to chromosome 14q11.2 based on an alignment of the HAUS4 sequence (GenBank AK000431) with the genomic sequence (GRCh37).

▼ REFERENCES

  1. Goshima, G., Mayer, M., Zhang, N., Stuurman, N., Vale, R. D. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J. Cell Biol. 181: 421-429, 2008. [PubMed: 18443220, images, related citations] [Full Text]

  2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/7/2010.

  3. Lawo, S., Bashkurov, M., Mullin, M., Gomez Ferreria, M., Kittler, R., Habermann, B., Tagliaferro, A., Poser, I., Hutchins, J. R. A., Hegemann, B., Pinchev, D., Buchholz, F., Peters, J.-M., Hyman, A. A., Gingras, A.-C., Pelletier, L. HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity. Curr. Biol. 19: 816-826, 2009. [PubMed: 19427217, related citations] [Full Text]

  4. Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R. D., Obuse, C., Goshima, G. The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc. Nat. Acad. Sci. 106: 6998-7003, 2009. [PubMed: 19369198, images, related citations] [Full Text]


Creation Date:
Patricia A. Hartz : 6/7/2010
Edit History:
mgross : 06/08/2010

* 613431

HAUS AUGMIN-LIKE COMPLEX, SUBUNIT 4; HAUS4


Alternative titles; symbols

CHROMOSOME 14 OPEN READING FRAME 94; C14ORF94


HGNC Approved Gene Symbol: HAUS4

Cytogenetic location: 14q11.2     Genomic coordinates (GRCh38): 14:22,946,228-22,957,090 (from NCBI)


TEXT

Description

HAUS4 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008; Uehara et al., 2009).


Cloning and Expression

Using mass spectrometry to identify proteins immunoprecipitated with the augmin complex subunit DGT6 (HAUS6; 613433) from HeLa cell mitotic extracts, Uehara et al. (2009) identified HAUS4, which they called C14ORF94. The deduced 363-amino acid protein has a calculated molecular mass of 42.4 kD.


Gene Function

Uehara et al. (2009) found that epitope-tagged HAUS4 immunoprecipitated endogenous DGT6 from HeLa cells. Like other augmin subunits, fluorescence-tagged HAUS4 localized to the metaphase spindle and accumulated at centrosomes during interphase. Knockdown of HAUS4 via RNA interference reduced the signals for gamma-tubulin (TUBG1; 191135) and microtubules at mitotic spindles.

Using RNA interference, Lawo et al. (2009) independently showed that HAUS4 is a critical component of the human augmin complex, and they confirmed interaction of HAUS4 with the other 7 augmin subunits by coimmunoprecipitation analysis. Knockdown of HAUS4 or any of the other augmin subunits resulted in destabilization of kinetochore microtubules, formation of multipolar spindles, fragmentation of centrosomes, and increased centrosome size. These severe mitotic defects were alleviated by codepletion of the nuclear mitotic apparatus (NUMA; see 164009), suggesting that augmin and NUMA regulate opposing activities.


Mapping

Hartz (2010) mapped the HAUS4 gene to chromosome 14q11.2 based on an alignment of the HAUS4 sequence (GenBank AK000431) with the genomic sequence (GRCh37).

REFERENCES

  1. Goshima, G., Mayer, M., Zhang, N., Stuurman, N., Vale, R. D. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J. Cell Biol. 181: 421-429, 2008. [PubMed: 18443220] [Full Text: https://doi.org/10.1083/jcb.200711053]

  2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/7/2010.

  3. Lawo, S., Bashkurov, M., Mullin, M., Gomez Ferreria, M., Kittler, R., Habermann, B., Tagliaferro, A., Poser, I., Hutchins, J. R. A., Hegemann, B., Pinchev, D., Buchholz, F., Peters, J.-M., Hyman, A. A., Gingras, A.-C., Pelletier, L. HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity. Curr. Biol. 19: 816-826, 2009. [PubMed: 19427217] [Full Text: https://doi.org/10.1016/j.cub.2009.04.033]

  4. Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R. D., Obuse, C., Goshima, G. The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc. Nat. Acad. Sci. 106: 6998-7003, 2009. [PubMed: 19369198] [Full Text: https://doi.org/10.1073/pnas.0901587106]


Creation Date:
Patricia A. Hartz : 6/7/2010

Edit History:
mgross : 06/08/2010



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: May 19, 2024