Alternative titles; symbols
HGNC Approved Gene Symbol: ARHGAP25
Cytogenetic location: 2p13.3 Genomic coordinates (GRCh38): 2:68,710,544-68,826,833 (from NCBI)
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004).
By sequencing clones obtained from a size-fractionated immature myeloid cell line cDNA library, Nomura et al. (1994) cloned ARHGAP25, which they designated KIAA0053. The deduced 638-amino acid protein shares similarity with CDC42GAP (ARHGAP1; 602732). Northern blot analysis detected highest expression in lung, spleen, and peripheral blood leukocytes. Expression was moderate in ovary and small intestine, weak in placenta, liver, skeletal muscle, kidney, thymus, and prostate, and not detected in heart, brain, pancreas, testis, and colon.
By searching an EST database for sequences similar to ARHGAP22 (610585), Katoh and Katoh (2004) identified 2 ARHGAP25 splice variants, 1 of which corresponds to the KIAA0053 cDNA cloned by Nomura et al. (1994). The other variant contains an alternate promoter and encodes a 639-amino acid protein that differs from the KIAA0053 protein at the N terminus. Both isoforms have an N-terminal pleckstrin homology domain, followed by a RhoGAP domain and a C-terminal coiled-coil domain.
Katoh and Katoh (2004) determined that the ARHGAP25 gene contains at least 12 exons, including 2 alternatively spliced first exons.
By PCR of a human/rodent hybrid panel, Nomura et al. (1994) mapped the ARHGAP25 gene to chromosome 2. Katoh and Katoh (2004) mapped the ARHGAP25 gene to chromosome 2p13 by genomic sequence analysis.
Katoh, M., Katoh, M. Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico. Int. J. Molec. Med. 14: 333-338, 2004. [PubMed: 15254788]
Nomura, N., Nagase, T., Miyajima, N., Sazuka, T., Tanaka, A., Sato, S., Seki, N., Kawarabayasi, Y., Ishikawa, K., Tabata, S. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 1: 223-229, 1994. [PubMed: 7584044] [Full Text: https://doi.org/10.1093/dnares/1.5.223]