Alternative titles; symbols
HGNC Approved Gene Symbol: ZFAND6
Cytogenetic location: 15q25.1 Genomic coordinates (GRCh38): 15:80,058,951-80,138,393 (from NCBI)
Using a yeast 2-hybrid screen of a HeLa cell library with the ubiquitin conjugating enzyme CDC34 (116948) as bait, Duan et al. (2000) isolated a novel cDNA clone which, after additional sequence analysis, matched 2 EST clones derived from normal human uterus and male retina. Duan et al. (2000) used these sequences to construct a full-length cDNA, designated AWP1 for 'associated with PRK1.' The deduced 208-amino acid AWP1 protein has a calculated molecular mass of 22.56 kD and shares approximately 55% sequence homology with mouse Awp1 and human ZNF216 (604761). AWP1 has 2 conserved zinc finger domains: ZFA20 at its N terminus and ZFAN1 at its C terminus. It contains 2 potential PEST sequences, 4 putative N-glycosylation sites, 7 casein kinase II phosphorylation sites, and 2 N-myristoylation sites. It has a putative nuclear localization signal, but no N-terminal signal peptide, transmembrane domain, or endoplasmic reticulum retention or membrane retention motifs. Northern blot analysis detected ubiquitous expression of a 1.5-kb transcript in all tissues examined, with relatively high levels in heart, skeletal muscle, liver, kidney, and placenta.
By in silico analysis, Duan et al. (2000) detected developmental expression of AWP1 in mRNA in an 8- to 9-week human fetus and in an 8-cell stage mouse embryo. Using ectopic expression of GST-tagged and Myc-tagged AWP1 mRNA in E. coli and COS-1 cells, respectively, Duan et al. (2000) determined that the recombinant AWP1 protein migrates at a molecular mass greater than that deduced from the amino acid sequence. Duan et al. (2000) concluded that AWP1 may form a dimer or, alternatively, may associate covalently with another protein.
Using coimmunoprecipitation and Western blot analysis, Duan et al. (2000) determined that mouse Awp1 associates with rat serine/threonine kinase Prk1 (PRKCL1; 601032) when cotransfected into COS-1 cells.
By sequence analysis, Duan et al. (2000) mapped the AWP1 gene to chromosome 15.
Duan, W., Sun, B., Li, T. W., Tan, B. J., Lee, M. K., Teo, T. S. Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo. Gene 256: 113-121, 2000. [PubMed: 11054541] [Full Text: https://doi.org/10.1016/s0378-1119(00)00365-6]