Alternative titles; symbols
HGNC Approved Gene Symbol: HUS1B
Cytogenetic location: 6p25.3 Genomic coordinates (GRCh38): 6:655,939-657,100 (from NCBI)
By database searching, Hang et al. (2002) identified HUS1B, a novel paralog of the human cell cycle checkpoint gene HUS1 (603760). HUS1B encodes a 278-amino acid protein that is 48% identical to the HUS1 protein. Hang et al. (2002) also identified mouse and rat orthologs of HUS1B. Northern blot analysis showed that expression of HUS1B in human tissues parallels that of HUS1. A 1.4-kb HUS1B transcript was observed in all tissues tested and an additional 1-kb band was observed in testis and prostate. Total HUS1B expression was highest in testis.
A HUS1-RAD1 (603153)-RAD9 (603761) protein complex is thought to form a proliferating cell nuclear antigen (PCNA; 176740)-like structure that is important for cell cycle checkpoint function. Using a yeast 2-hybrid analysis, Hang et al. (2002) showed that whereas HUS1 can bind RAD1, RAD9, and another molecule of HUS1, HUS1B directly interacts with RAD1 but not with RAD9 or HUS1, suggesting that HUS1B cannot simply substitute for HUS1 in the protein complex. Overexpression of HUS1B but not HUS1 in human 293T cells led to clonogenic cell death. Hang et al. (2002) suggested that HUS1B and HUS1 have distinct but related roles in regulating cell cycle checkpoints and genomic integrity.
Hang et al. (2002) determined that the HUS1B gene is intronless.
By sequence analysis, Hang et al. (2002) mapped the HUS1B gene to chromosome 6p25.3-p25.1.
Hang, H., Zhang, Y., Dunbrack, R. L., Jr., Wang, C., Lieberman, H. B. Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1. Genomics 79: 487-492, 2002. [PubMed: 11944979] [Full Text: https://doi.org/10.1006/geno.2002.6737]