Alternative titles; symbols
HGNC Approved Gene Symbol: POTEE
Cytogenetic location: 2q21.1 Genomic coordinates (GRCh38): 2:131,209,536-131,265,278 (from NCBI)
Bera et al. (2002) identified 10 paralogs of the POTE gene (607549), including POTE2. By partially sequencing clones obtained from prostate and testis cDNA libraries, they determined that the POTE2 gene is transcribed.
By PCR, Bera et al. (2004) cloned 4 full-length splice variants of POTE2 from normal prostate cDNA. The longest deduced protein, POTE2A, contains 712 amino acids and has a calculated molecular mass of 72.5 kD. POTE2A has 3 cysteine-rich N-terminal repeats, followed by 7 ankyrin repeats and a C-terminal helical region. The deduced 394-amino acid POTE2B protein has a calculated molecular mass of 40.2 kD. Compared with POTE2A, POTE2B has extra sequence within the ankyrin repeat region and lacks the C-terminal helical region. POTE2C and POTE2D differ only in the long 3-prime UTR. The deduced 376-amino acid POTE2C/POTE2D protein has a calculated molecular mass of 38.6 kD. Compared with POTE2A, POTE2C/POTE2D lacks the C-terminal helical region. In vitro transcription and translation of the POTE2 variants resulted in proteins with molecular masses in agreement with their calculated masses.
By genomic sequence analysis, Hahn et al. (2006) identified 5 POTE2 genes, which they called POTE2-alpha, -beta, -gamma, -delta, and -beta-prime, on chromosome 2. POTE2-beta and -beta-prime are identical. The POTE2 splice variants reported by Bera et al. (2004) are encoded by the POTE2-gamma gene.
Based on sequence similarity, the POTE genes can be divided into 3 groups. Lee et al. (2006) determined that members of group 3, which includes the POTE2 genes, have an insertion of a beta-actin (102630) retrogene at their 3-prime ends, just prior to a LINE element that is present in all POTE genes. The actin sequence is included within the ORFs of POTE2-alpha, POTE2-beta, and POTE2-gamma. In the other members of group 3, a stop codon before the actin module results in premature termination.
Bera et al. (2004) determined that the POTE2 gene has 15 exons and spans about 45 kb.
By genomic sequence analysis, Bera et al. (2002) mapped paralogs of the POTE gene to chromosomes 2q22.2 and 2q14.3. Using FISH, they mapped POTE2 to chromosome 2q13-q22.
By genomic sequence analysis, Hahn et al. (2006) identified 5 POTE2 genes within a 2-Mb region of chromosome 2q21.1. Two of the genes, POTE2-beta and -beta-prime, are identical and are embedded in a long palindromic segment.
Lee et al. (2006) reported that the POTE genes are found only in primates. They determined that the POTE genes entered the primate genome before the divergence of New World monkeys and Old World monkeys. A beta-actin sequence was inserted at the 3-prime end of an ancestral POTE gene before the divergence of Old World monkeys and apes and is now present in several POTE paralogs, including the POTE2 genes.
Bera, T. K., Huynh, N., Maeda, H., Sathyanarayana, B. K., Lee, B., Pastan, I. Five POTE paralogs and their splice variants are expressed in human prostate and encode proteins of different lengths. Gene 337: 45-53, 2004. [PubMed: 15276201] [Full Text: https://doi.org/10.1016/j.gene.2004.05.009]
Bera, T. K., Zimonjic, D. B., Popescu, N. C., Sathyanarayana, B. K., Kumar, V., Lee, B., Pastan, I. POTE, a highly homologous gene family located on numerous chromosomes and expressed in prostate, ovary, testis, placenta, and prostate cancer. Proc. Nat. Acad. Sci. 99: 16975-16980, 2002. Note: Erratum: Proc. Nat. Acad. Sci. 100: 462 only, 2003. [PubMed: 12475935] [Full Text: https://doi.org/10.1073/pnas.262655399]
Hahn, Y., Bera, T. K., Pastan, I. H., Lee, B. Duplication and extensive remodeling shaped POTE family genes encoding proteins containing ankyrin repeat and coiled coil domains. Gene 366: 238-245, 2006. [PubMed: 16364570] [Full Text: https://doi.org/10.1016/j.gene.2005.07.045]
Lee, Y., Ise, T., Ha, D., Saint Fleur, A., Hahn, Y., Liu, X.-F., Nagata, S., Lee, B., Bera, T. K., Pastan, I. Evolution and expression of chimeric POTE-actin genes in the human genome. Proc. Nat. Acad. Sci. 103: 17885-17890, 2006. [PubMed: 17101985] [Full Text: https://doi.org/10.1073/pnas.0608344103]