Entry - *608887 - PURINE-RICH ELEMENT-BINDING PROTEIN B; PURB - OMIM

 
 
* 608887

PURINE-RICH ELEMENT-BINDING PROTEIN B; PURB


Alternative titles; symbols

PUR-BETA


HGNC Approved Gene Symbol: PURB

Cytogenetic location: 7p13     Genomic coordinates (GRCh38): 7:44,876,299-44,885,530 (from NCBI)


TEXT

▼ Cloning and Expression

By probing a HeLa cell cDNA library with a fragment of the PURA (600473) sequence, Bergemann et al. (1992) identified a partial sequence of a homologous cDNA, designated PURB, encoding a protein with a 23-amino acid class I motif and an amphipathic helix similar to those found in PURA.

Kelm et al. (1997) cloned mouse Purb (p44) and Pura (p46) and identified them as the 2 components of the previously designated vascular actin single-stranded DNA-binding factor-2, which specifically bound to purine-rich regions within an enhancer and an exon of vascular actin (Kelm et al., 1996). Like Pura, the deduced 324-amino acid Purb protein contains class I and class II repeats and a 23-amino acid PSYC motif that is homologous to the Rb-binding region of SV-40 large T antigen. Purb differs from Pura in that the second class II repeat is interrupted by a glycine-rich region, the N-terminal glycine-rich region is interrupted by a 6-amino acid region, and there is no glutamine-rich C-terminal region.


▼ REFERENCES

  1. Bergemann, A. D., Ma, Z.-W., Johnson, E. M. Sequence of cDNA comprising the human PUR gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein. Molec. Cell. Biol. 12: 5673-5682, 1992. [PubMed: 1448097, related citations] [Full Text]

  2. Kelm, R. J., Jr., Elder, P. K., Strauch, A. R., Getz, M. J. Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Pur-alpha and Pur-beta. J. Biol. Chem. 272: 26727-26733, 1997. [PubMed: 9334258, related citations] [Full Text]

  3. Kelm, R. J., Jr., Sun, S., Strauch, A. R., Getz, M. J. Repression of transcriptional enhancer factor-1 and activator protein-1-dependent enhancer activity by vascular actin single-stranded DNA binding factor 2. J. Biol. Chem. 271: 24278-24285, 1996. [PubMed: 8798674, related citations] [Full Text]


Creation Date:
Laura L. Baxter : 8/30/2004
Edit History:
carol : 09/01/2004
carol : 8/31/2004

* 608887

PURINE-RICH ELEMENT-BINDING PROTEIN B; PURB


Alternative titles; symbols

PUR-BETA


HGNC Approved Gene Symbol: PURB

Cytogenetic location: 7p13     Genomic coordinates (GRCh38): 7:44,876,299-44,885,530 (from NCBI)


TEXT

Cloning and Expression

By probing a HeLa cell cDNA library with a fragment of the PURA (600473) sequence, Bergemann et al. (1992) identified a partial sequence of a homologous cDNA, designated PURB, encoding a protein with a 23-amino acid class I motif and an amphipathic helix similar to those found in PURA.

Kelm et al. (1997) cloned mouse Purb (p44) and Pura (p46) and identified them as the 2 components of the previously designated vascular actin single-stranded DNA-binding factor-2, which specifically bound to purine-rich regions within an enhancer and an exon of vascular actin (Kelm et al., 1996). Like Pura, the deduced 324-amino acid Purb protein contains class I and class II repeats and a 23-amino acid PSYC motif that is homologous to the Rb-binding region of SV-40 large T antigen. Purb differs from Pura in that the second class II repeat is interrupted by a glycine-rich region, the N-terminal glycine-rich region is interrupted by a 6-amino acid region, and there is no glutamine-rich C-terminal region.


REFERENCES

  1. Bergemann, A. D., Ma, Z.-W., Johnson, E. M. Sequence of cDNA comprising the human PUR gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein. Molec. Cell. Biol. 12: 5673-5682, 1992. [PubMed: 1448097] [Full Text: https://doi.org/10.1128/mcb.12.12.5673-5682.1992]

  2. Kelm, R. J., Jr., Elder, P. K., Strauch, A. R., Getz, M. J. Sequence of cDNAs encoding components of vascular actin single-stranded DNA-binding factor 2 establish identity to Pur-alpha and Pur-beta. J. Biol. Chem. 272: 26727-26733, 1997. [PubMed: 9334258] [Full Text: https://doi.org/10.1074/jbc.272.42.26727]

  3. Kelm, R. J., Jr., Sun, S., Strauch, A. R., Getz, M. J. Repression of transcriptional enhancer factor-1 and activator protein-1-dependent enhancer activity by vascular actin single-stranded DNA binding factor 2. J. Biol. Chem. 271: 24278-24285, 1996. [PubMed: 8798674] [Full Text: https://doi.org/10.1074/jbc.271.39.24278]


Creation Date:
Laura L. Baxter : 8/30/2004

Edit History:
carol : 09/01/2004
carol : 8/31/2004



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: May 9, 2024