Alternative titles; symbols
HGNC Approved Gene Symbol: TUBE1
Cytogenetic location: 6q21 Genomic coordinates (GRCh38): 6:112,070,663-112,087,482 (from NCBI)
Chang and Stearns (2000) identified TUBE within a genomic database based on its sequence homology with other tubulin genes, and they cloned the full-length cDNA by PCR of a HEK293 cell cDNA library. The deduced 475-amino acid protein has a calculated molecular mass of 53 kD. TUBE contains the conserved GTP-binding domain shared with other tubulins, but it lacks the highly acidic C terminus characteristic of alpha-tubulin (see 602529) and beta-tubulin (see 191130). Relative to other tubulins, it also has a 26-amino acid insertion between helices 6 and 7. Localization of TUBE within the centrosome of dividing osteogenic sarcoma cells was dependent on the cell-cycle stage. In cells with only 1 centrosome, TUBE roughly colocalized with gamma-tubulin (see 191135), but in cells with duplicated centrosomes, TUBE often labeled only 1 of the pair, with variable labeling of the second centrosome. Gamma-tubulin homogeneously labeled both. Chang and Stearns (2000) determined that TUBE associates with the older of the 2 centrosomes in a newly duplicated pair and later associates with both.
By genomic sequence analysis, Chang and Stearns (2000) mapped the TUBE1 gene to chromosome 6q21.
Chang, P., Stearns, T. Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function. Nature Cell Biol. 2: 30-35, 2000. [PubMed: 10620804] [Full Text: https://doi.org/10.1038/71350]