HGNC Approved Gene Symbol: SEC24A
Cytogenetic location: 5q31.1 Genomic coordinates (GRCh38): 5:134,648,382-134,727,909 (from NCBI)
In yeast, the Sec23-Sec24 complex is a component of coat protein II (COPII; see 601924)-coated vesicles that mediate protein transport from the endoplasmic reticulum. SEC24A is 1 of several mammalian proteins that show structural and functional homology to yeast Sec24.
By searching an EST database for homologs of yeast Sec24 proteins, screening a size-selected B-lymphocyte cDNA library, and 5-prime RACE, Pagano et al. (1999) isolated a cDNA encoding SEC24A. The deduced 1,078-amino acid protein has a calculated molecular mass of about 118 kD. Pagano et al. (1999) noted, however, that the sequence is likely incomplete because it lacks the initiator ATG. RNase protection assays demonstrated that SEC24A, SEC24B (607184), and SEC24C (607185) were expressed simultaneously in several cell lines, including lines originating from fibroblasts, hepatocytes, and lymphocytes. The major difference in expression was a constant 2- to 3-fold lower level of expression of SEC24B compared with SEC24A and SEC24C.
Tang et al. (1999) determined that SEC24A and SEC24B share about 56% sequence identity, while SEC24C and SEC24D (607186) share about 52% identity. However, the identity shared between these 2 pairs is only about 20%.
The International Radiation Hybrid Mapping Consortium mapped the SEC24A gene to chromosome 5 (stSG9839).
Pagano, A., Letourneur, F., Garcia-Estefania, D., Carpentier, J.-L., Orci, L., Paccaud, J.-P. Sec24 proteins and sorting at the endoplasmic reticulum. J. Biol. Chem. 274: 7833-7844, 1999. [PubMed: 10075675] [Full Text: https://doi.org/10.1074/jbc.274.12.7833]
Tang, B. L., Kausalya, J., Low, D. Y. H., Lock, M. L., Hong, W. A family of mammalian proteins homologous to yeast Sec24p. Biochem. Biophys. Res. Commun. 258: 679-684, 1999. [PubMed: 10329445] [Full Text: https://doi.org/10.1006/bbrc.1999.0574]