Alternative titles; symbols
HGNC Approved Gene Symbol: TNFAIP2
Cytogenetic location: 14q32.32 Genomic coordinates (GRCh38): 14:103,121,469-103,137,439 (from NCBI)
TNF (191160) is a proinflammatory cytokine with pleiotropic effects on cells and tissues, mediated mostly by alterations in target tissue gene expression. Using differential hybridization to identify primary response genes induced by TNF in human umbilical vein endothelial cells (HUVECs), Sarma et al. (1992) isolated a HUVEC cDNA encoding TNFAIP2. Northern blot analysis detected a 4.2-kb human TNFAIP2 transcript. During mouse development, expression of the Tnfaip2 transcript varied in a temporal fashion that was organ-specific. The TNFAIP2 cDNA encodes a predicted 654-amino acid protein with a single N-linked glycosylation site; stretches of glutamates, lysines, and alanines near the N terminus; and 4 glutamines near the C terminus. Immunoprecipitation studies confirmed the calculated molecular mass of 73 kD. Sarma et al. (1992) used Southern blot analysis to show that TNFAIP2 is an evolutionarily conserved single-copy gene.
Sarma et al. (1992) found that the TNFAIP2 gene was induced in HUVECs in response to the proinflammatory molecules TNF, IL1B (147720), and lipopolysaccharide. TNFAIP2 demonstrated the characteristics of a primary response gene in its rapid and substantial induction in the absence of intermediary protein synthesis. The authors found that TNFAIP2 transcription was induced during capillary tube formation in vitro.
By somatic cell hybrid analysis and fluorescence in situ hybridization, Sarma et al. (1992) localized the TNFAIP2 gene to chromosome 14q32.
Sarma, V., Wolf, F. W., Marks, R. M., Shows, T. B., Dixit, V. M. Cloning of a novel tumor necrosis factor-alpha-inducible primary response gene that is differentially expressed in development and capillary tube-like formation in vitro. J. Immun. 148: 3302-3312, 1992. [PubMed: 1374453]