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| Status |
Public on Apr 26, 2015 |
| Title |
Transcriptomics Analyses of soybean leaves and roots sample during water-deficit |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray experiments were performed on the roots and leaves samples seperately using custom based Nimblgen platform (12plex). This is a time-course study. The plants were grown in controlled growth chamber condiditons ambiient to soybean growth and development using a hydrophonics system. The tissues were harvested after 30days of growth in six time points- 0min, 30mins, 1hour, 2hours, 3hours and 5hours of dehydration.
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| Overall design |
We used a hydrophonics system for the trancriptomics analysis of the soybean(William -82) root and leaves samples to study the reponse during water- deficit or drought. Plants were grown in controlled growth chamber conditions and harvested after 30days in six time-points. The plants were transferred to empty pots without touching to nullify any possibility of wounding and harvested subsequently. For 0mins, plants were taken out of the well-watered conditions and transfered to empty boxes and immediately harvested for both tisses and hence called 0-min dehydrated tissue. Similarly other time-points were processed (for instance, 2hrs of dehydration, plants were dehydrated for 2hours after being taken out from well-waterted pots and then harvested). Tissues for three biological replicates and three plants per biological replicates were collected for each time-point (n=9). RNA was islolated from both tissues using RNeasy Kit from Qiagen and quality was accessed with Nano-drop as well as with Agilent-Bioanalyzer using RNA-600 kit. 20ug of sample was processed at Micro Array Facility.
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| Contributor(s) |
Tripathi P, Rabara RC, Rushton PJ |
| Citation(s) |
26123791, 26861168 |
| Submission date |
Aug 05, 2013 |
| Last update date |
Oct 16, 2019 |
| Contact name |
Paul J Rushton |
| E-mail(s) |
paul.rushton@tamu.edu
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| Phone |
(972) 952 9246
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| Organization name |
Texas A&M AgriLife Research & Extension Center
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| Street address |
17360 Coit Road
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| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75252 |
| Country |
USA |
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| Platforms (1) |
| GPL17531 |
NimbleGen Glycine Max Array [100526_Brach_MoGene_exp] |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA214193 |
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