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| Status |
Public on Aug 22, 2011 |
| Title |
Testosterone-dependent and independent transcriptional networks in the hypothalamus of Gpr54 and Kiss1 knockout male mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Humans and mice with loss of function mutations in GPR54 (KISS1R) or kisspeptin (KISS1) do not progress through puberty, caused by a failure to release GnRH. The transcriptional networks regulated by these proteins in the hypothalamus have yet to be explored by genome-wide methods.
Using micro-dissected hypothalamic tissues to isolate RNA from our mice, we first set out to define the transcriptional differences among the wild type and knockout mice. Since the GPR54-kisspeptin axis is subject to hormonal feedback and the knockout mice are pre-pubertal, we also tested the hormonal dependence/independence of each differentially expressed transcript. To avoid variation in gene expression due to fluctuations in the levels of circulating hormones during the female estrous cycle, only male mice were used in this study.
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| Overall design |
17 samples were successfully hybridized; 4 GKO, 5 KKO, 3 WT 3 weeks old, 5 WT 6 weeks old. We compared GKO to KKO, GKO to WT, KKO to WT and all KO to all WT.
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| Contributor(s) |
Prentice LM, d’Anglemont de Tassigny X, McKinney S, Ruiz de Algara T, Yap D, Turashvili G, Poon S, Sutcliffe M, Allard P, Burleigh A, Fee J, Huntsman DG, Colledge WH, Aparicio SA |
| Citation(s) |
21527035 |
| Submission date |
Apr 05, 2011 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Steven McKinney |
| E-mail(s) |
smckinney@bccrc.ca
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| Phone |
604-675-8000
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| Organization name |
British Columbia Cancer Agency
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| Department |
Molecular Oncology
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| Lab |
Samuel Aparicio
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| Street address |
675 West 10th Ave
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| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
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| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA139091 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28383.xls.gz |
267.6 Kb |
(ftp)(http) |
XLS |
| GSE28383_RAW.tar |
383.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data not provided for this record |
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