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Series GSE17820 Query DataSets for GSE17820
Status Public on Feb 15, 2010
Title HuR differentially regulates unique subsets of mRNAs in estrogen receptor negative and positive breast cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Analysis of RNA immunoprecipitation of HuR, a RNA binding protein (RBP), in breast cancer cell lines. This approach, utilizing RNA immunoprecipitation hybridized to microarray (RIP-Chip), provides global identification of putative endogenous mRNA targets of different RBPs. HuR is an RBP that binds to the AU-rich (ARE) regions of labile mRNAs, such as proto-oncogenes, facilitating their translation into protein. HuR has been shown to play a role in cancer progression and elevated levels of cytoplasmic HuR directly correlate with increased invasiveness and poor prognosis for many cancers, including those of the breast. We used HuR RIP-Chip as a comprehensive and systematic method to survey breast cancer target genes in both MCF-7 (estrogen receptor positive, ER+) and MDA-MB-231 (estrogen receptor negative, ER-) breast cancer cell lines. We identified unique subsets of HuR associated mRNAs found individually or in both cell types. Two novel HuR targets, CD-9 and CALM-2, were identified and validated by quantitative RT-PCR and biotin pulldown analysis. Our findings reveal that the differential regulation of these two cancer-related genes by HuR was contingent upon the cellular environment.
 
Overall design RNA immunoprecipitation of the HuR RNA binding protein by 3A2 antibody and IgG (control) from two human breast cancer cell lines, MCF-7 and MDA-MB-231 .
 
Contributor(s) Atasoy U, Davis JW, Calaluce R, Grubin M, Magee J, Kuwano Y, Gorospe M
Citation(s) 20370918
Submission date Aug 26, 2009
Last update date Jan 08, 2019
Contact name J Wade Davis
Organization name AbbVie
Street address 1 N Waukegan Rd
City North Chicago
State/province IL
ZIP/Postal code 60048
Country USA
 
Platforms (1)
GPL7363 Illumina HumanWG-6_V2_0_R2
Samples (12)
GSM444627 MDA 3A2 pulldown rep1
GSM444628 MCF 3A2 pulldown rep1
GSM444629 MCF IgG pulldown rep1
Relations
BioProject PRJNA120039

Download family Format
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MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17820_non-normalized.txt.gz 7.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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