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Series GSE70670 Query DataSets for GSE70670
Status Public on Sep 30, 2016
Title Distinct transcriptome responses to water limitation in isohydric and anisohydric grapevine cultivars
Organism Vitis vinifera
Experiment type Expression profiling by array
Summary Grapevine (Vitis vinifera L.) is an economically important crop with a wide geographical distribution, reflecting its ability to grow successfully in a range of climates. However, many vineyards are located in regions with seasonal drought, and these are often predicted to be global climate change hotspots. Climate change affects the entire physiology of grapevine, with strong effects on yield, wine quality and typicity, making it difficult to produce berries of optimal enological quality and consistent stability over the forthcoming decades.
Here we investigated the reactions of two grapevine cultivars to water stress, the isohydric variety Montepulciano and the anisohydric variety Sangiovese, by examining physiological and molecular perturbations in the leaf and berry. A multidisciplinary approach was used to characterize the distinct stomatal behavior of the two cultivars and its impact on leaf and berry gene expression. Positive associations were found among the photosynthetic, physiological and transcriptional modifications, and candidate genes encoding master regulators of the water stress response were identified using an integrated approach based on the analysis of topological co-expression network properties. In particular, the genome-wide transcriptional study indicated that the isohydric behavior relies upon the following responses: i) faster transcriptome response after stress imposition; ii) faster abscisic acid-related gene modulation; iii) more rapid expression of heat shock protein (HSP) genes and iv) reversion of gene-expression profile at rewatering. Conversely, that reactive oxygen species (ROS)-scavenging enzymes, molecular chaperones and abiotic stress-related genes were induced earlier and more strongly in the anisohydric cultivar.
Overall, the present work found original evidence of a molecular basis for the proposed classification between isohydric and anisohydric grapevine genotypes.
 
Overall design Two, six and 27 days after water deprivation (three time points), three pools of leaves and three pools of berries were sampled per every date at midday (11.0-12.00 hours) from control and treated vines of both Sangiovese and Montepulciano cultivars, entailing 36 leaf and 36 berry samples. Moreover, biological triplicates of leaves were collected after re-watering (46 days after stress imposition) from control and recovered vines of both Sangiovese and Montepulciano cultivars, entailing 12 samples. The entire microarray experiment comprises a total of 84 samples.
 
Contributor(s) Dal Santo S, Paliotti A, Zenoni S, Tornielli G, Fasoli M, Paci P, Tombesi S, Frioni T, Silvestroni O, Bellincontro A, D'Onofrio C, Matarese F, Gatti M, Poni S, Pezzotti M
Citation(s) 27765014
Submission date Jul 09, 2015
Last update date Jan 09, 2017
Contact name Marianna Fasoli
E-mail(s) marianna.fasoli@univr.it
Phone +39 045 842 5625
Organization name University of Verona
Department Biotechnology
Lab Molecular Viticulture
Street address Via della Pieve 70
City San Pietro in Cariano
State/province Verona
ZIP/Postal code 37029
Country Italy
 
Platforms (1)
GPL17894 NimbleGen 090918 Vitus vinifera exp HX12 [090918_Vitus_exp; GENE_CALL_ID version]
Samples (84)
GSM1816199 1_Leaves_Sangiovese_Control_Sampling1
GSM1816200 2_Leaves_Sangiovese_Control_Sampling1
GSM1816201 3_Leaves_Sangiovese_Control_Sampling1
Relations
BioProject PRJNA289331

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70670_RAW.tar 189.3 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
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