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Series GSE51326 Query DataSets for GSE51326
Status Public on Jan 21, 2014
Title Assessment of hematopoietic failure due to RPL11 deficiency in a zebrafish model of Diamond–Blackfan anemia by deep sequencing
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary To comprehensively reflect the impact of RPL11 deficiency on the transcriptome of zebrafish embryos, we collected 40–50 RPL11-deficient and MO control zebrafish embryos at 48 hpf from separate experiments and constructed two mRNA-seq sequencing libraries in parallel. High-throughput sequencing was performed on the Hi-Seq2000 sequencing platform in parallel. The number of sequenced gene transcript reads was 35–40 million. We found that hemoglobin biosynthetic and hematological defects in RPL11-deficient zebrafish were related to dysregulation of iron metabolism-related genes, including tfa, tfr1b, alas2 and slc25a37, which are involved in heme and hemoglobin biosynthesis. In addition, we found reduced expression of the hematopoietic stem cells (HSC) marker c-myb and HSC transcription factor tal1 and hoxb4a in RPL11-deficient zebrafish embryos, indicating that the hematopoietic defects may be related to impaired HSC differentiation and proliferation. However, RPL11 deficiency did not affect the development of other blood cell lineages such as granulocytes and myelocytes.
 
Overall design Compare 2 different transcriptomes of RPL11-deficient and MO control zebrafish embryos
 
Contributor(s) Fang X
Citation(s) 24341334, 26961822, 26484089
Submission date Sep 30, 2013
Last update date Jun 07, 2019
Contact name yadong yang
E-mail(s) yangyd@big.ac.cn
Organization name Beijing Institute of Genomics
Street address No.1-104 Beichen West Road, Chaoyang
City Beijing
ZIP/Postal code 100101
Country China
 
Platforms (1)
GPL14875 Illumina HiSeq 2000 (Danio rerio)
Samples (2)
GSM1242917 morpholino-control
GSM1242918 RPL11-MO
Relations
BioProject PRJNA222400
SRA SRP030657

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51326_RAW.tar 840.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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