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Series GSE37476 Query DataSets for GSE37476
Status Public on Mar 30, 2013
Title Time course of gene expression changes after muscle contraction in spinal cord injured rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Purpose: The goal of this study was to determine the gene expression changes that occur over 7 days in parralyzed muscle in response to isometric contraction elicited by electrical stimulation initiated 4 months after spinal cord injury and to compare such changes to those observed in a normal muscle subjected to overload.
 
Overall design Methods: Electrical stimulation of the soleus and plantaris muscle was stimulated in female rats with complete transection of the spinal cord at the interspace between the 9th and 10th thoracic vertebrae. Stimulation was begun 16 weeks after spinal cord transection and produced near-isometric contraction of soleus, plantaris and tibialis anterior. Muscle was analyzed at 1, 2 and 7 days after starting exercise with electrical stimulation. To provide a baseline reference for gene expression at 16 weeks after spinal cord injury, muscle was also analysed from an additional group of spinal cord transected animals. One additional group of animals with a sham-spinal cord injury was included to provide information about gene expression in neurologically intact animals of similar age. In parallel studies, rats underwent bilateral gastrocnemius ablation to overload soleus and plantaris, or a sham ablation as a control. Muscle was analyzed at 1, 3 and 7 days after gastrocnemius ablation or sham-ablation.
Gene expression was determined using Affymetrix Rat Exon microarrays. For each group of animals, microarray analysis was performed for soleus muscle for each of 3 separate animals, using one array per animal. Control sammples for the spinal cord injured groups included a group of animals with a Sham-spinal cord injury, and a group of spinal cord injured animals that did not get electrical stimulation. The comparator for determining fold-change expression values was the spinal cord injured group that did not receive electrical stimulation. For each day after gastrocnemius ablation, a control was included that received all procedures needed for this ablation except cutting the distal insertion of the gastrocnemius into the Achilles tendon to control for effects of the surgery on gene expression.
 
Contributor(s) Wu Y, Collier L, Qin W, Creasy G, Bauman WA, Jarvis J, Cardozo C
Citation(s) 23914941
Submission date Apr 21, 2012
Last update date Jun 27, 2014
Contact name christopher p cardozo
E-mail(s) chris.cardozo@mssm.edu
Phone 718-584-9000
Organization name Bronx VA Medical Center
Department CMSCI
Lab Room 3F-35
Street address 130 West Kingsbridge Road
City Bronx
State/province NY
ZIP/Postal code 10468
Country USA
 
Platforms (1)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
Samples (33)
GSM920760 SCI-Sham_ES-1
GSM920761 SCI-ES-1Day-1
GSM920762 SCI-ES-1Day-2
Relations
BioProject PRJNA162663

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37476_RAW.tar 135.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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