|
Status |
Public on Feb 23, 2010 |
Title |
Non adherent macrophage of mafb null and WT |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
WT macrophage
|
Organism |
Mus musculus |
Characteristics |
developmental stage: E14.5 tissue: fetal liver cell type: macrophage culture condition: M-CSF induced in non adherent culture dish genome/variation: wild type
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy mini Kit (Qiagen)
|
Label |
Cy3
|
Label protocol |
Low RNA Input Linear Amplification Kit(Agilent)、Cyanine3-CTP&Cyanine5-CTP(PerkinElmer)
|
|
|
Channel 2 |
Source name |
Mafb null macrophage
|
Organism |
Mus musculus |
Characteristics |
developmental stage: E14.5 tissue: fetal liver cell type: macrophage culture condition: M-CSF induced in non adherent culture dish genome/variation: Mafb null
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy mini Kit (Qiagen)
|
Label |
Cy5
|
Label protocol |
Low RNA Input Linear Amplification Kit(Agilent)、Cyanine3-CTP&Cyanine5-CTP(PerkinElmer)
|
|
|
|
Hybridization protocol |
Agilent Mouse Oligo Microarray Kit(G4121A)
|
Scan protocol |
Agilent G2565AA microarray scanner
|
Description |
Data was analyzed by Excel software
|
Data processing |
Data were analyzed by Feature Extraction Software Version A.7.5.1 using linear and lowess normalization methods. Additional processing/filtering was carried out using Microsoft Excel.
|
|
|
Submission date |
Feb 18, 2010 |
Last update date |
Feb 23, 2010 |
Contact name |
Michito Hamada |
E-mail(s) |
michit-hamada06@ob.md.tsukuba.ac.jp
|
Phone |
81-29-853-6963
|
Organization name |
University of Tsukuba
|
Department |
anatomy and embryogy
|
Street address |
1-1-1 tennodai
|
City |
TSUKUBA |
State/province |
IBARAKI |
ZIP/Postal code |
305-8575 |
Country |
Japan |
|
|
Platform ID |
GPL891 |
Series (1) |
GSE20419 |
Non adherent macrophage of mafb null and WT |
|