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Sample GSM511101 Query DataSets for GSM511101
Status Public on Feb 23, 2010
Title Non adherent macrophage of mafb null and WT
Sample type RNA
 
Channel 1
Source name WT macrophage
Organism Mus musculus
Characteristics developmental stage: E14.5
tissue: fetal liver
cell type: macrophage
culture condition: M-CSF induced in non adherent culture dish
genome/variation: wild type
Extracted molecule total RNA
Extraction protocol RNeasy mini Kit (Qiagen)
Label Cy3
Label protocol Low RNA Input Linear Amplification Kit(Agilent)、Cyanine3-CTP&Cyanine5-CTP(PerkinElmer)
 
Channel 2
Source name Mafb null macrophage
Organism Mus musculus
Characteristics developmental stage: E14.5
tissue: fetal liver
cell type: macrophage
culture condition: M-CSF induced in non adherent culture dish
genome/variation: Mafb null
Extracted molecule total RNA
Extraction protocol RNeasy mini Kit (Qiagen)
Label Cy5
Label protocol Low RNA Input Linear Amplification Kit(Agilent)、Cyanine3-CTP&Cyanine5-CTP(PerkinElmer)
 
 
Hybridization protocol Agilent Mouse Oligo Microarray Kit(G4121A)
Scan protocol Agilent G2565AA microarray scanner
Description Data was analyzed by Excel software
Data processing Data were analyzed by Feature Extraction Software Version A.7.5.1 using linear and lowess normalization methods. Additional processing/filtering was carried out using Microsoft Excel.
 
Submission date Feb 18, 2010
Last update date Feb 23, 2010
Contact name Michito Hamada
E-mail(s) michit-hamada06@ob.md.tsukuba.ac.jp
Phone 81-29-853-6963
Organization name University of Tsukuba
Department anatomy and embryogy
Street address 1-1-1 tennodai
City TSUKUBA
State/province IBARAKI
ZIP/Postal code 305-8575
Country Japan
 
Platform ID GPL891
Series (1)
GSE20419 Non adherent macrophage of mafb null and WT

Data table header descriptions
ID_REF
VALUE Agilent Feature Extraction (7.5.1) normalized signal intensity ratio Cy5/Cy3 (Mafb null/wild type)
gProcessedSignal WT macrophage signal
rProcessedSignal MafbKO macrophage signal
gMeanSignal WT macrophage Meansignal

Data table
ID_REF VALUE gProcessedSignal rProcessedSignal gMeanSignal
3 9.069465901e-002 2.555500e+002 3.148984e+002 1.452459e+002
4 2.819181489e-002 2.522166e+002 2.691320e+002 1.449298e+002
5 2.067332994e-003 6.125193e+003 6.154419e+003 9.363684e+002
6 1.496137753e-001 3.622108e+002 5.111816e+002 1.595932e+002
8 1.003084036e-001 3.399135e+002 4.282297e+002 1.568000e+002
9 0.000000000e+000 2.421688e+001 4.102410e+001 1.118393e+002
10 5.508263473e-002 1.570209e+002 1.782544e+002 1.328361e+002
11 3.278914652e-002 1.563883e+003 1.686527e+003 3.195556e+002
12 8.426510023e-002 1.189968e+002 1.444776e+002 1.279661e+002
13 7.751666705e-003 1.039178e+003 1.057893e+003 2.501034e+002
15 2.248556495e-002 3.162619e+004 3.330676e+004 4.412141e+003
16 6.674819240e-002 4.702731e+001 5.484011e+001 1.187049e+002
17 3.101896723e-002 2.570897e+003 2.761237e+003 4.548983e+002
18 -3.977275645e-002 9.074283e+003 8.280176e+003 1.340397e+003
19 -1.422600379e-002 1.951492e+004 1.888603e+004 2.770086e+003
20 1.399642129e-002 1.963520e+003 2.027831e+003 3.735263e+002
22 -1.311456553e-002 3.339807e+002 3.240461e+002 1.570678e+002
23 9.326220453e-003 6.295764e+002 6.432424e+002 1.964364e+002
24 4.302133014e-002 5.832947e+003 6.440348e+003 8.983729e+002
25 -6.590444101e-002 1.136956e+002 9.768754e+001 1.282712e+002

Total number of rows: 21318

Table truncated, full table size 1353 Kbytes.




Supplementary file Size Download File type/resource
GSM511101.txt.gz 5.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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