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Status |
Public on Jul 06, 2016 |
Title |
Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population |
Organism |
Aedes aegypti |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. The emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and Zika. Therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. Among the new approaches implemented for pest control, one of the most promising is RNA interference (RNAi). The aim of this study was to provide a feasible RNAi solution that can be applied on wild pyrethroid resistant mosquito populations in the near future. To achieve this, high dsRNA efficacy at economic quantities is required. It is recognized that the sodium channel transcript variability governs its functional diversity including the emergence of insecticide resistance. Therefore, to maximize the RNAi effect, we tiled a number of overlapping dsRNA constructs that together target about half of the voltage-gated sodium channel (VGSC) transcript variants annotated in this work. This strategy provided a refined dsRNA trigger that increased mortality with a three-fold decrease in dsRNA amounts compared to the primary VGSC dsRNA construct. Thus, we demonstrated the use of RNA interference (RNAi) to increase susceptibility of adult mosquitoes to a widely used pyrethroid insecticide.
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Overall design |
Small RNA sequences from 5 mosquitoes treated with Random or VGSC dsRNAs were generated using Illumina HiSeq 2500.
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Contributor(s) |
Dalla Bona AC, Chitolina RF, Lopes Fermino M, de Castro Poncio L, Weiss A, Pereira Lima JB, Paldi N, Soares Bernardes E, Henen J, Maori E |
Citation(s) |
27416771 |
Submission date |
Jul 05, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Eyal Maori |
E-mail(s) |
eyal@forrestinnovations.com
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Phone |
+44-7778-633-526
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Organization name |
Forrest Innovations Ltd
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Department |
N/A
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Lab |
N/A
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Street address |
17 Alon Hatavor street POB 3164
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City |
Caesarea |
State/province |
N/A |
ZIP/Postal code |
3088900 |
Country |
Israel |
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Platforms (1) |
GPL19664 |
Illumina HiSeq 2500 (Aedes aegypti) |
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Samples (2) |
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Relations |
BioProject |
PRJNA327841 |
SRA |
SRP077923 |
Supplementary file |
Size |
Download |
File type/resource |
GSE84030_RAW.tar |
60.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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