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Series GSE72108 Query DataSets for GSE72108
Status Public on Feb 10, 2017
Title Boron-deficiency-responsive microRNAs and their targets in Citrus sinensis leaves
Organism Citrus sinensis
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Background: MicroRNAs play important roles in the adaptive responses of plants to nutrient deficiencies. Here, we sequenced two small RNA libraries from B-deficient and -sufficient (control) Citrus sinensis leaves, respectively, using Illumina sequencing in order to identify the potential miRNAs related to the tolerance of citrus to B-deficiency. Results: Ninety one (83 known and 8 novel) up- and 81 (75 known and 6 novel) downregulated miRNAs were isolated from B-deficient leaves. The great alteration of miRNA expression might contribute to the tolerance of citrus to B-deficiency. The adaptive responses of miRNAs to B-deficiency might related to several aspects: (a) attenuation of plant growth and development by repressing auxin signaling due to decreased TIR1 level and ARF-mediated gene expression by altering the expression of miR393, miR160 and miR3946; (b) maintaining leaf phenotype and enhancing the stress tolerance by up-regulating NACs targeted by miR159, miR782, miR3946 and miR7539; (c) activation of the stress responses and antioxidant system through down-regulating the expression of miR164, miR6260, miR5929, miR6214, miR3946 and miR3446; (d) decreasing the expression of major facilitator superfamily protein genes targeted by miR5037, thus lowering B export from plants. Also, B-deficiency-induced downregulation of miR408 might play a role in plant tolerance to B-deficiency by regulating Cu homeostasis and enhancing superoxide dismutase activity. Conclusions: Our study reveals some novel responses of citrus to B-deficiency, which increase our understanding of the adaptive mechanisms of citrus to B-deficiency at the miRNA (post-transcriptional) level.
 
Overall design Two libraries were constructed from C. sinensis leaves subjected to 0(B-deficient, Xa) or 10(B-sufficient,control, Xb) μM B for 15 weeks, and sequenced by Illumina sequencing. Size fractionated small RNAs (16-30 bp) from total RNA extracts was ligated to 5' and 3' adapters, and reverse transcribed. After PCR amplification the sample was subjected to Solexa sequencing. The clean tags were used for further analysis.
 
Contributor(s) Lu Y, Qi Y
Citation(s) 26538180
Submission date Aug 16, 2015
Last update date May 15, 2019
Contact name Chen Lisong
E-mail(s) lisongchen@fafu.edu.cn
Phone +8613599390418
Organization name Fujian Agriculture and Forestry University
Street address Shangdian street NO.15
City Fuzhou
ZIP/Postal code 350002
Country China
 
Platforms (1)
GPL9256 Illumina Genome Analyzer (Citrus sinensis)
Samples (2)
GSM1855471 Xa leaves
GSM1855472 Xb leaves
Relations
BioProject PRJNA292994
SRA SRP062451

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE72108_RAW.tar 67.6 Mb (http)(custom) TAR (of TXT)
GSE72108_miR_count.sig.txt.gz 21.1 Kb (ftp)(http) TXT
GSE72108_miR_count.std.txt.gz 8.6 Kb (ftp)(http) TXT
GSE72108_miR_count.summary.txt.gz 6.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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