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Status |
Public on Apr 06, 2016 |
Title |
SpdR regulon |
Platform organism |
Caulobacter vibrioides CB15 |
Sample organism |
Caulobacter vibrioides NA1000 |
Experiment type |
Expression profiling by array
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Summary |
As bacterial cells enter stationary phase, they adjust their growth rate to comply with nutrient restriction and acquire increased resistance to several stresses. These events are regulated by controlling gene expression at this phase, changing the mode of exponential growth into that of growth arrest, and increasing the expression of proteins involved in stress resistance. The two-component system SpdR/SpdS is required for the activation of transcription of the Caulobacter crescentus cspD gene at the onset of stationary phase. A Global transcriptional analysis at early stationary phase of a spdR null mutant strain compared to the wild type strain was carried out by DNA microarray. Twenty-three genes showed at least twofold decreased expression in the spdR deletion mutant strain relative to its parental strain, including cspD, while five genes showed increased expression in the mutant. The expression of a set of ten genes was evaluated by quantitative real time PCR, validating the microarray data. Several of the differentially expressed genes can be involved in modulating gene expression, including four transcriptional regulators, and the RNA regulatory protein Hfq. The ribosomal proteins NusE and NusG, which also that have additional regulatory functions in transcription and translation, were also downregulated in the spdR mutant, as well as the ParE1 toxin. The results indicate that SpdR regulates several genes encoding proteins of regulatory function, which in turn may be required for the expression of other genes important for the transition to stationary phase.
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Overall design |
Four replicates perfomed with independent biological samples
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Contributor(s) |
da Silva CA, Lourenco RF, Mazzon RR, Ribeiro RA, Marques MV |
Citation missing |
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Submission date |
Jul 24, 2015 |
Last update date |
Apr 09, 2016 |
Contact name |
Rogerio Lourenco |
E-mail(s) |
lourenco@iq.usp.br
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Organization name |
Universidade de Sao Paulo
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Department |
Bioquimica
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Street address |
Av. Prof. Lineu Prestes, 748
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City |
Sao Paulo |
State/province |
Sao Paulo |
ZIP/Postal code |
05508-900 |
Country |
Brazil |
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Platforms (1) |
GPL10469 |
MIT Agilent Caulobacter 45K Array |
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Samples (4)
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Relations |
BioProject |
PRJNA290917 |