NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE71146 Query DataSets for GSE71146
Status Public on Jul 22, 2015
Title Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions
Organism Vitis vinifera
Experiment type Expression profiling by high throughput sequencing
Summary To obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in China, next-generation sequencing technology was performed on E-L 31, 35,36 and 38 stages of Muscat Blanc a Petits Grains grape berries from Changli (CL, eastern) and Gaotai (GT, western). Transcriptome analysis revealed that some key genes involved in terpene synthesis were markedly up-regulated in the CL region. Particularly in the MEP pathway, the expression of VvHDR (1-hydroxy-2-methyl-2-butenyl 4-diphosphate reductase) paralleled with the accumulation of terpenes, which can promote the flow of isopentenyl diphosphate (IPP) into the terpene synthetic pathway. The glycosidically bound monoterpenes accumulated differentially along with maturation in both regions, which is synchronous with the expression of a monoterpene glucosyltransferase gene (VvGT14). Other genes were also found to be related to the differential accumulation of terpenes and monoterpene glycosides in the grapes between regions. Transcription factors that could regulate terpene synthesis were predicted through gene co-expression network analysis. Additionally, the genes involved in abscisic acid (ABA) and ethylene signal responses were expressed at high levels earlier in GT grapes than in CL grapes.
 
Overall design cDNA libraries generated from four developmental stages (E-L 31, 35, 36 and 38) of Muscat Blanc a Petits Grains grape berries in two consecutive years from Changli (CL) and Gaotai (GT) in China were sequenced using Illumina HiSeq™ 2000.Only one RNA-seq library was constructed for each of the E-L31 and E-L36 stage samples because of the small amount of high quality RNA acquired, whereas two libraries were constructed for each of the E-L35 and E-L38 stage samples. As a result, a total of 24 libraries were obtained for the two regions within two years.
 
Contributor(s) Wen Y, Zhong G, Gao Y, Duan C, Pan Q
Citation(s) 26444528
Submission date Jul 21, 2015
Last update date Apr 01, 2020
Contact name Qiu-hong Pan
E-mail(s) panqh@cau.edu.cn
Organization name China Agriculture University
Department College of Food Science and Nutritional Engineering
Lab Centre for Viticulture and Enology
Street address Tsinghua East Road No.17,Haidian district
City Beijing
ZIP/Postal code 100083
Country China
 
Platforms (1)
GPL18740 Illumina HiSeq 2000 (Vitis vinifera)
Samples (16)
GSM1828478 2010C1
GSM1828479 2010C2
GSM1828480 2010C3
Relations
BioProject PRJNA290459
SRA SRP061365

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE71146_RAW.tar 10.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap