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Status |
Public on Apr 18, 2016 |
Title |
Transcriptomic and metabolomic profiling of ionic liquid stimuli unveils enhanced secondary metabolism in Aspergillus nidulans |
Platform organisms |
Aspergillus nidulans FGSC A4; Neurospora crassa OR74A |
Sample organism |
Aspergillus nidulans |
Experiment type |
Expression profiling by array
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Summary |
The study aims essentially in the analysis of the transcriptomic and metabolomic profiles induced by the presence of the tested ionic liquids in the metabolism of Aspergillus nidulans. Focusing specially on the secondary metabolism, which genes are clustered.
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Overall design |
Aspergillus nidulans cultures were grown for fifteen days in mineral media alone (control) or supplemented with 1-ethyl-3-methylimidazolium chloride or cholinium chloride at a concentration of half the MIC previously determined for 1-ethyl-3-methylimidazolium chloride, namely 0.7 M for A. nidulans. To investigate the impact of the tested ionic liquids in the metabolism of A. nidulans, the fungal transcriptome (three biological replicates) was analysed using Affymetrix microarrays. Pair-wise comparisons were used to identify the set of genes with altered expression levels between the control and the media supplemented with either 1-ethyl-3-methylimidazolium chloride or cholinium chloride after fifteen days.
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Contributor(s) |
Pereira CS, Becker JD, Alves PC, Hartmann DO, Martins I, Garcia H |
Citation(s) |
27072538 |
Submission date |
Feb 14, 2015 |
Last update date |
Apr 18, 2016 |
Contact name |
Cristina Silva Pereira |
E-mail(s) |
spereira@itqb.unl.pt
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Organization name |
Instituto de Tecnologia Química e Biológica (ITQB)
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Lab |
Applied and Environmental Mycology Laboratory (AEM)
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Street address |
Rua da Quinta Grande nº6
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City |
Lisboa |
ZIP/Postal code |
2780-156 Oeiras |
Country |
Portugal |
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Platforms (1) |
GPL18227 |
FungiANC (GeneChip FungiANCa520710F) |
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Samples (9)
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GSM1611572 |
Aspergillus nidulans grown in glucose media for fifteen days, biological rep. 1 |
GSM1611573 |
Aspergillus nidulans grown in glucose media for fifteen days, biological rep. 2 |
GSM1611574 |
Aspergillus nidulans grown in glucose media for fifteen days, biological rep. 3 |
GSM1611575 |
Aspergillus nidulans grown in 0.7M of cholinium chloride media for fifteen days, biological rep. 1 |
GSM1611576 |
Aspergillus nidulans grown in 0.7M of cholinium chloride media for fifteen days, biological rep. 2 |
GSM1611577 |
Aspergillus nidulans grown in 0.7M of cholinium chloride media for fifteen days, biological rep. 3 |
GSM1611578 |
Aspergillus nidulans grown in 0.7M of 1-ethyl-3-methylimidazolium chloride media for fifteen days, biological rep. 1 |
GSM1611579 |
Aspergillus nidulans grown in 0.7M of 1-ethyl-3-methylimidazolium chloride media for fifteen days, biological rep. 2 |
GSM1611580 |
Aspergillus nidulans grown in 0.7M of 1-ethyl-3-methylimidazolium chloride media for fifteen days, biological rep. 3 |
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Relations |
BioProject |
PRJNA275466 |