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| Status |
Public on Dec 12, 2014 |
| Title |
Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection [life cycle] |
| Organism |
Phytophthora infestans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Background: The oomycete Phytophthora infestans possesses active RNA silencing pathways, which presumably enable this plant pathogen to control the large numbers of transposable elements present in its 240 Mb genome. Small RNAs (sRNAs), central molecules in RNA silencing, are known to also play key roles in this organism, notably in regulation of critical effector genes needed for infection of its potato host. Results: In order to identify additional classes of sRNAs in oomycetes, we mapped deep sequencing reads to transfer RNAs (tRNAs) thereby revealing the presence of 19-40 nt tRNA-derived RNA fragments (tRFs). Northern blot analysis identified abundant tRFs corresponding to half tRNA molecules. Some tRFs accumulated differentially during infection, as seen by examining sRNAs sequenced from P. infestans-potato interaction libraries. The putative connection between tRF biogenesis and the canonical RNA silencing pathways was investigated by employing hairpin RNA-mediated RNAi to silence the genes encoding P. infestans Argonaute (PiAgo) and Dicer (PiDcl) endoribonucleases. By sRNA sequencing we show that tRF accumulation is PiDcl1-independent, while Northern hybridizations detected reduced levels of specific tRNA-derived species in the PiAgo1 knockdown line. Conclusions: Our findings extend the sRNA diversity in oomycetes to include fragments derived from non-protein-coding RNA transcripts and identify tRFs whose levels are elevated during infection of P. infestans on potato.
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| Overall design |
Small RNA sequence data from two Phytophthora infestans isolates. Four life-cycle stages from each isolate. No replicates. Total number of samples: 8.
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| Contributor(s) |
Åsman AK, Vetukuri RR, Jahan SN, Fogelqvist J, Corcoran P, Avrova AO, Whisson SC, Dixelius C |
| Citation(s) |
25492044 |
| Submission date |
Oct 24, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Anna Åsman |
| E-mail(s) |
anna.asman@slu.se
|
| Organization name |
Swedish University of Agricultural Sciences (SLU)
|
| Department |
Molecular Sciences
|
| Street address |
PO Box 7080
|
| City |
Uppsala |
| ZIP/Postal code |
SE-750 07 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL19354 |
AB SOLiD System 2.0 (Phytophthora infestans) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE63293 |
Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection |
|
| Relations |
| BioProject |
PRJNA264780 |
| SRA |
SRP049232 |
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