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Status |
Public on Jun 01, 2014 |
Title |
Expression data from Arabidopsis flowers under moderate drought stress |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
We also used microarray analysis to examine transcriptomic changes under moderate drought, identifying thousends of genes that potentially mediate moderate drought responses in the flower, including genes encoding transcription factors that likely play crucial regulatory roles.
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Overall design |
Arabidopsis were well-watered until after just bolting (after 24 days growth with the main stem about 1 cm high) when moderate drought treatment was started by withholding water (defined as day 0 for moderate drought treatment). The relative soil moisture content decreased rapidly and, after about 48 hours the relative soil moisture content was near 50% of the soil water-holding capacity (first moderate drough treated sample M3 were collected at day 3 (72 h after withholding water)). The soil water condition was maintained by daily watering until almost all the fruits were mature and ready to harvest (about 50 days). For well-watered (control) plants, 90% of the soil water-holding capacity was maintained until tissue harvest or after seed maturation (pots were weighed and watered twice per day). Unopened floral bud samples were collected at day 0, 3, 4, 5, 10.
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Contributor(s) |
Ma X, Su Z, Ma H |
Citation(s) |
24928551 |
Submission date |
Feb 27, 2014 |
Last update date |
Jun 12, 2017 |
Contact name |
Zhao SU |
Organization name |
The Penn State University
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Department |
Biology
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Lab |
Sarah Assmann
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Street address |
354 N. Frear
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City |
University Park |
State/province |
PA |
ZIP/Postal code |
16802 |
Country |
USA |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (10)
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GSM1336520 |
Wild type (Col-0) inflorescence, well-watered, day 0, replicate 1 [C01] |
GSM1336521 |
Wild type (Col-0) inflorescence, well-watered, day 0, replicate 2 [C02] |
GSM1336522 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 3, replicate 1 |
GSM1336523 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 3, replicate 2 |
GSM1336524 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 4, replicate 1 |
GSM1336525 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 4, replicate 2 |
GSM1336526 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 5, replicate 1 |
GSM1336527 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 5, replicate 2 |
GSM1336528 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 10, replicate 1 |
GSM1336529 |
Wild type (Col-0) inflorescence, moderate drought-treated, day 10, replicate 2 |
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Relations |
BioProject |
PRJNA239569 |
Supplementary file |
Size |
Download |
File type/resource |
GSE55431_RAW.tar |
20.1 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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