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Series GSE54366 Query DataSets for GSE54366
Status Public on Dec 14, 2014
Title Analysis of the transcriptional responses in inflorescence buds of Jatropha curcas exposed to cytokinin treatment
Organism Jatropha curcas
Experiment type Expression profiling by array
Summary Jatropha curcas L. is a potential biofuel plant. Application of exogenous cytokinin (6-benzyladenine, BA) on its inflorescence buds can significantly increase the number of female flowers, thereby improving seed yield. To investigate which genes and signal pathways are involved in the response to cytokinin in J. curcas inflorescence buds, we monitored transcriptional activity in inflorescences at 0, 3, 12, 24, and 48 h after BA treatment using a microarray. We detected 5,555 differentially expressed transcripts over the course of the experiment, which could be grouped into 12 distinct temporal expression patterns. We also identified 31 and 131 transcripts in J. curcas whose homologs in model plants function in flowering and phytohormonal signaling pathways, respectively. According to the transcriptional analysis of genes involved in flower development, we hypothesized that BA treatment delays floral organ formation by inhibiting the transcription of the A, B and E classes of floral organ-identity genes, which would allow more time to generate more floral primordia in inflorescence meristems, thereby enhancing inflorescence branching and significantly increasing flower number per inflorescence. BA treatment might also play an important role in maintaining the flowering signals by activating the transcription of GIGANTEA (GI) and inactivating the transcription of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and TERMINAL FLOWER 1b (TFL1b). In addition, exogenous cytokinin treatment could regulate the expression of genes involved in the metabolism and signaling of other phytohormones, indicating that cytokinin and other phytohormones jointly regulate flower development in J. curcas inflorescence buds. Our study provides a framework to better understand the molecular mechanisms underlying changes in flowering traits in response to cytokinin treatment in J. curcas inflorescence buds. The results provide valuable information related to the mechanisms of cross-talk among multiple phytohormone signaling pathways in woody plants.
 
Overall design Gene expression of flower buds in response to BA was measured at 0h, 3h, 12h, 24h and 48h after 1mM 6-benzylaminopurine treatment. Three biological replicates were prepared for each time point.
 
Contributor(s) Xu ZF, Chen MS, Pan BZ, Wang GJ, Ni J, Niu LJ
Citation(s) 25433671
Submission date Jan 23, 2014
Last update date Oct 02, 2015
Contact name MaoSheng - Chen
E-mail(s) chenms@xtbg.org.cn
Phone +86-0691-8713053
Organization name Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences
Department Molecular breeding of energy plants
Lab Laboratory of Tropical Plant Resource Science
Street address Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences
City Mengla
State/province Yunnan
ZIP/Postal code 666303
Country China
 
Platforms (1)
GPL18092 Agilent-030273 Jatropha curcas 4X44k micrarray (probe name version)
Samples (15)
GSM1313925 0h-1-1
GSM1313926 0h-1-2
GSM1313927 0h-1-3
Relations
BioProject PRJNA236337

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54366_RAW.tar 34.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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