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Status |
Public on Dec 14, 2014 |
Title |
Analysis of the transcriptional responses in inflorescence buds of Jatropha curcas exposed to cytokinin treatment |
Organism |
Jatropha curcas |
Experiment type |
Expression profiling by array
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Summary |
Jatropha curcas L. is a potential biofuel plant. Application of exogenous cytokinin (6-benzyladenine, BA) on its inflorescence buds can significantly increase the number of female flowers, thereby improving seed yield. To investigate which genes and signal pathways are involved in the response to cytokinin in J. curcas inflorescence buds, we monitored transcriptional activity in inflorescences at 0, 3, 12, 24, and 48 h after BA treatment using a microarray. We detected 5,555 differentially expressed transcripts over the course of the experiment, which could be grouped into 12 distinct temporal expression patterns. We also identified 31 and 131 transcripts in J. curcas whose homologs in model plants function in flowering and phytohormonal signaling pathways, respectively. According to the transcriptional analysis of genes involved in flower development, we hypothesized that BA treatment delays floral organ formation by inhibiting the transcription of the A, B and E classes of floral organ-identity genes, which would allow more time to generate more floral primordia in inflorescence meristems, thereby enhancing inflorescence branching and significantly increasing flower number per inflorescence. BA treatment might also play an important role in maintaining the flowering signals by activating the transcription of GIGANTEA (GI) and inactivating the transcription of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and TERMINAL FLOWER 1b (TFL1b). In addition, exogenous cytokinin treatment could regulate the expression of genes involved in the metabolism and signaling of other phytohormones, indicating that cytokinin and other phytohormones jointly regulate flower development in J. curcas inflorescence buds. Our study provides a framework to better understand the molecular mechanisms underlying changes in flowering traits in response to cytokinin treatment in J. curcas inflorescence buds. The results provide valuable information related to the mechanisms of cross-talk among multiple phytohormone signaling pathways in woody plants.
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Overall design |
Gene expression of flower buds in response to BA was measured at 0h, 3h, 12h, 24h and 48h after 1mM 6-benzylaminopurine treatment. Three biological replicates were prepared for each time point.
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Contributor(s) |
Xu ZF, Chen MS, Pan BZ, Wang GJ, Ni J, Niu LJ |
Citation(s) |
25433671 |
Submission date |
Jan 23, 2014 |
Last update date |
Oct 02, 2015 |
Contact name |
MaoSheng - Chen |
E-mail(s) |
chenms@xtbg.org.cn
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Phone |
+86-0691-8713053
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Organization name |
Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences
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Department |
Molecular breeding of energy plants
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Lab |
Laboratory of Tropical Plant Resource Science
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Street address |
Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences
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City |
Mengla |
State/province |
Yunnan |
ZIP/Postal code |
666303 |
Country |
China |
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Platforms (1) |
GPL18092 |
Agilent-030273 Jatropha curcas 4X44k micrarray (probe name version) |
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Samples (15)
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Relations |
BioProject |
PRJNA236337 |
Supplementary file |
Size |
Download |
File type/resource |
GSE54366_RAW.tar |
34.3 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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