|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Mar 08, 2013 |
Title |
Selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine. |
Organism |
Vitis vinifera |
Experiment type |
Expression profiling by array
|
Summary |
The purpose of this study was to quantify the effects of basal leaf removal applied in Sangiovese cultivar at two different phonological stages, pre-bloom and veraison, on berry composition. As very few papers were published about the regulation of gene expression induced by vineyard management techniques, we report the first global transcriptomic analysis (integrated with agronomic and biochemical data) aiming to determine the molecular mechanisms that control Sangiovese berry composition. The comparison of gene expression profiles of defoliated vines at pre-bloom and at veraison with the control, revealed a common transcriptional response at the end of veraison in both treated berries, but also a more extensive transcriptome rearrangement in pre-bloom defoliated ones, which could be linked to the strong biochemical changes detected in the berry after pre-bloom veraison.
|
|
|
Overall design |
Total RNA recovered from a pool of berries derived from three control plants (C) was compared to total RNA from a pool of berries derived from three defoliated vines at pre-bloom (PB, JD 147,6 basal leaves and relative laterals removed from each shoot) and three defoliated vines at veraison (V, JD 211,6 basal leaves and relative laterals removed from each shoot) at beginning of veraison (BV), end of veraison (EV) and harvest (H). For each sampling date microarray analyses were conducted for three different biological replicates for treatment.
|
|
|
Contributor(s) |
Pastore C, Zenoni S |
Citation(s) |
23433030 |
Submission date |
Aug 30, 2012 |
Last update date |
Feb 26, 2016 |
Contact name |
Chiara Pastore |
Organization name |
University of Bologna
|
Street address |
Viale Fanin, 46
|
City |
Bologna |
ZIP/Postal code |
40126 |
Country |
Italy |
|
|
Platforms (1) |
GPL13936 |
NimbleGen 090918 Vitus vinifera exp HX12 [090918_Vitus_exp] |
|
Samples (24)
|
GSM884850 |
control, untreated, replicate 1, end of véraison |
GSM884851 |
control, untreated, replicate 2, end of véraison |
GSM884852 |
control, untreated, replicate 3, end of véraison |
GSM884856 |
control, untreated, replicate 1, harvest |
GSM884857 |
control, untreated, replicate 2, harvest |
GSM884858 |
control, untreated, replicate 3, harvest |
GSM994874 |
control, untreated, replicate 1, beginning of veraison |
GSM994875 |
control, untreated, replicate 2, beginning of veraison |
GSM994876 |
control, untreated, replicate 3, beginning of veraison |
GSM994877 |
pre-bloom defoliation, replicate 1, beginning of veraison |
GSM994878 |
pre-bloom defoliation, replicate 2, beginning of veraison |
GSM994879 |
pre-bloom defoliation, replicate 3, beginning of veraison |
GSM994880 |
pre-bloom defoliation, replicate 1, end of veraison |
GSM994881 |
pre-bloom defoliation, replicate 2, end of veraison |
GSM994882 |
pre-bloom defoliation, replicate 3, end of veraison |
GSM994883 |
veraison defoliation, replicate 1, end of veraison |
GSM994884 |
veraison defoliation, replicate 2, end of veraison |
GSM994885 |
veraison defoliation, replicate 3, end of veraison |
GSM994886 |
pre-bloom defoliation, replicate 1, harvest |
GSM994887 |
pre-bloom defoliation, replicate 2, harvest |
GSM994888 |
pre-bloom defoliation, replicate 3, harvest |
GSM994889 |
veraison defoliation, replicate 1, harvest |
GSM994890 |
veraison defoliation, replicate 2, harvest |
GSM994891 |
veraison defoliation, replicate 3, harvest |
|
Relations |
BioProject |
PRJNA174145 |
Supplementary file |
Size |
Download |
File type/resource |
GSE40487_RAW.tar |
60.0 Mb |
(http)(custom) |
TAR (of PAIR) |
Processed data included within Sample table |
|
|
|
|
|