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Series GSE36194 Query DataSets for GSE36194
Status Public on May 09, 2012
Title North American Brain Expression Consortium: DNA Methylation
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary A fundamental challenge in the post-genome era is to understand and annotate the consequences of genetic variation, particularly within the context of human tissues. We describe a set of integrated experiments designed to investigate the effects of common genetic variability on DNA methylation and mRNA expression distinct human brain regions. We show that brain tissues may be readily distinguished based on methylation status or expression profile. We find an abundance of genetic cis regulation mRNA expression and show for the first time abundant quantitative trait loci for DNA CpG methylation. We observe that the largest magnitude effects occur across distinct brain regions. We believe these data, which we have made publicly available, will be useful in understanding the biological effects of genetic variation.

Authorized Access data:
Mapping of GEO sample accessions to dbGaP subject/sample IDs is available through dbGaP Authorized Access, see http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/projects/gap/cgi-bin/study.cgi?study_id=phs000249
 
Overall design Because of our interest in genomic regulation of expression and neurological disorders we embarked upon a series of experiments to provide a brain region-specific contextual framework for genetic and epigenetic regulation of gene expression. We obtained frozen brain tissue from the cerebellum and frontal cortex from 318 subjects (total 724 tissue samples).
 
Contributor(s) Gibbs JR, Hernandez DG, Dillman A, Traynor BJ, Nalls MA, Arepalli S, van der Brug MP, Troncoso J, Johnson R, O'Brien R, Zielke HR, Zonderman A, Ferrucci L, Longo DL, Walker R, Cookson MR, Singleton AB
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Submission date Mar 01, 2012
Last update date Jan 02, 2015
Contact name J Raphael Gibbs
E-mail(s) gibbsr@mail.nih.gov
Organization name National Institutue on Aging, NIH
Department Computational Biology Core
Lab Laboratory of Neurogenetics
Street address 35 Convent Drive, Bldg 35/1A1015, MSC3707
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL8490 Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)
Samples (724)
GSM401538 UMARY-914-CRBLM-CpG
GSM401539 UMARY-5087-CRBLM-CpG
GSM401540 JHU-384-CRBLM-CpG
Relations
BioProject PRJNA164721

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36194_RAW.tar 5.8 Mb (http)(custom) TAR
Processed data included within Sample table

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