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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jun 04, 2011 |
Title |
Expression data from mouse B16-F10 cells treated with heat shock (HS), Lipofectamine 2000 (LA), and benzyl alcohol (BA) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Three types of stimuli -- heat shock, Lipofectamine 2000 and benzyl alcohol -- induce activity of some stress genes (hsp) in mouse B16-F10 cells. Besides hsp genes induction, each stimulus causes gene expression changes of different sets of genes. We used microarrays to analyze global gene expression changes in mouse B16-F10 cells treated with elevated temperature (heat shock, HS), with Lipofectamine 2000 (LA) or with 40mM benzyl alcohol (BA).
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Overall design |
In order to study how lipofection may affect cellular homeostasis, we used Affymetrix microarrays to analyze the whole transcriptome of mouse B16-F10 cells treated with Lipofectamine 2000. To find out which genes are affected, we compared the cells treated with Lipofectamine (LA1, LA2, LA3), the cells treated with 40mM benzyl alcohol (BA1, BA2, BA3), heat-shocked cells (HS1, HS2, HS3) and control, untreated cells (C1-5). RNA was extracted from cells 30 min after treatment.
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Contributor(s) |
Lisowska K |
Citation(s) |
21663599 |
Submission date |
Jun 03, 2011 |
Last update date |
Jan 08, 2019 |
Contact name |
Katarzyna Marta Lisowska |
E-mail(s) |
klisowska@io.gliwice.pl
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Organization name |
MSC Memorial Cancer Center and Institute of Oncology, Gliwice Branch
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Department |
Center for Translational Research and Molecular Biology of Cancer
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Street address |
ul. Wybrzeże Armii Krajowej 15
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City |
Gliwice |
ZIP/Postal code |
44-101 |
Country |
Poland |
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Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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Samples (14)
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GSM737424 |
control B16-F10 cells, biological rep1 |
GSM737425 |
control B16-F10 cells, biological rep2 |
GSM737426 |
control B16-F10 cells, biological rep3 |
GSM737427 |
control B16-F10 cells, biological rep4 |
GSM737428 |
control B16-F10 cells, biological rep5 |
GSM737429 |
heat-shocked B16-F10 cells, biological rep1 |
GSM737430 |
heat-shocked B16-F10 cells, biological rep2 |
GSM737431 |
heat-shocked B16-F10 cells, biological rep3 |
GSM737432 |
B16-F10 cells treated with Lipofectamine, biological rep1 |
GSM737433 |
B16-F10 cells treated with Lipofectamine, biological rep2 |
GSM737434 |
B16-F10 cells treated with Lipofectamine, biological rep3 |
GSM737435 |
B16-F10 cells treated with 40 mM benzyl alcohol, biological rep1 |
GSM737436 |
B16-F10 cells treated with 40 mM benzyl alcohol, biological rep2 |
GSM737437 |
B16-F10 cells treated with 40 mM benzyl alcohol, biological rep3 |
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Relations |
BioProject |
PRJNA141173 |
Supplementary file |
Size |
Download |
File type/resource |
GSE29743_RAW.tar |
32.1 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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