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Series GSE29743 Query DataSets for GSE29743
Status Public on Jun 04, 2011
Title Expression data from mouse B16-F10 cells treated with heat shock (HS), Lipofectamine 2000 (LA), and benzyl alcohol (BA)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Three types of stimuli -- heat shock, Lipofectamine 2000 and benzyl alcohol -- induce activity of some stress genes (hsp) in mouse B16-F10 cells. Besides hsp genes induction, each stimulus causes gene expression changes of different sets of genes. We used microarrays to analyze global gene expression changes in mouse B16-F10 cells treated with elevated temperature (heat shock, HS), with Lipofectamine 2000 (LA) or with 40mM benzyl alcohol (BA).
 
Overall design In order to study how lipofection may affect cellular homeostasis, we used Affymetrix microarrays to analyze the whole transcriptome of mouse B16-F10 cells treated with Lipofectamine 2000. To find out which genes are affected, we compared the cells treated with Lipofectamine (LA1, LA2, LA3), the cells treated with 40mM benzyl alcohol (BA1, BA2, BA3), heat-shocked cells (HS1, HS2, HS3) and control, untreated cells (C1-5). RNA was extracted from cells 30 min after treatment.
 
Contributor(s) Lisowska K
Citation(s) 21663599
Submission date Jun 03, 2011
Last update date Jan 08, 2019
Contact name Katarzyna Marta Lisowska
E-mail(s) klisowska@io.gliwice.pl
Organization name MSC Memorial Cancer Center and Institute of Oncology, Gliwice Branch
Department Center for Translational Research and Molecular Biology of Cancer
Street address ul. Wybrzeże Armii Krajowej 15
City Gliwice
ZIP/Postal code 44-101
Country Poland
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (14)
GSM737424 control B16-F10 cells, biological rep1
GSM737425 control B16-F10 cells, biological rep2
GSM737426 control B16-F10 cells, biological rep3
Relations
BioProject PRJNA141173

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29743_RAW.tar 32.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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