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| Status |
Public on Aug 18, 2011 |
| Title |
dRNA-Seq of Chlamydia pneumoniae |
| Organism |
Chlamydia pneumoniae CWL029 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The obligate intracellular human pathogen Chlamydia pneumoniae was subjected to dRNA-Seq to gain insights into the transcriptome. The two distinct life cycle forms elementary bodies (EB) and reticulate bodies (RB) were isolated from human Hep2 cell line by differential gradient centrifugation.
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| |
|
| Overall design |
Total RNA was isolated and partially treated with Terminator Exonuclease to digest RNA without 5'-PPP and thereby enrich for native 5' ends.
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| |
|
| Contributor(s) |
Albrecht M, Sharma CM, Reinhardt R, Vogel J, Rudel T |
| Citation(s) |
21989159 |
| Submission date |
Oct 28, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Marco Albrecht |
| E-mail(s) |
marco.albrecht@uni-wuerzburg.de
|
| Phone |
+49 931 3184413
|
| Fax |
+49 931 3184402
|
| Organization name |
University of Würzburg
|
| Department |
Department of Microbiology
|
| Lab |
Rudel lab
|
| Street address |
Am Hubland
|
| City |
Würzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL11140 |
454 GS (Chlamydophila pneumoniae CWL029) |
|
| Samples (8)
|
|
| Relations |
| SRA |
SRP004041 |
| BioProject |
PRJNA131985 |
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