NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE17117 Query DataSets for GSE17117
Status Public on Jul 16, 2009
Title Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush
Organism Mus musculus
Experiment type Expression profiling by array
Summary Retinal ganglion cell (RGC) death is the final consequence of many blinding diseases, where there is considerable variation in the time course and severity of RGC loss. Indeed, this process appears to be influenced by a wide variety of genetic and environmental factors. In this study we explored the genetic basis for differences in ganglion cell death in two inbred strains of mice. We found that RGCs are more susceptible to death following optic nerve crush in C57BL/6J mice (54% survival) than in DBA2/J mice (62% survival). Using the Illumina Mouse-6 microarray, we identified 1,580 genes with significant change in expression following optic nerve crush in these two strains of mice. Our analysis of the changes occurring after optic nerve crush demonstrated that the greatest amount of change (44% of the variance) was due to the injury itself. This included changes associated with ganglion cell death, reactive gliosis, and abortive regeneration. The second pattern of gene changes (23% of the variance) was primarily related to differences in gene expressions observed between the C57BL/6J and DBA/2J mouse strains. The remaining changes in gene expression represent interactions between the effects of optic nerve crush and the genetic background of the mouse. We extracted one genetic network from this dataset that appears to be related to tissue remodeling. One of the most intriguing sets of changes included members of the crystallin family of genes, which may represent a signature of pathways modulating the susceptibility of cells to death. Differential responses to optic nerve crush between two widely used strains of mice were used to define molecular networks associated with ganglion cell death and reactive gliosis. These results form the basis for our continuing interest in the modifiers of retinal injury.
 
Overall design 18 Samples: 9 per strain (C57BL/6J & DBA/2J); 3 conditions per strain
 
Contributor(s) Templeton JP, Nassr M, Vazquez-Chona F, Freeman-Anderson NE, Orr WE, Williams RW, Geisert EE
Citation(s) 19643015
Submission date Jul 15, 2009
Last update date Mar 21, 2012
Contact name William Edwin Orr
E-mail(s) weorr@utmem.edu
Phone 901 448-3635
Fax 901 448-5028
Organization name University of Tennessee Health Science Center
Department Ophthalmology
Lab Vision Core
Street address 930 Madison Avenue, Suite 757
City Memphis
State/province TN
ZIP/Postal code 38163
Country USA
 
Platforms (1)
GPL4234 Sentrix Mouse-6 Expression BeadChip
Samples (18)
GSM428128 Retina C57BL/6J control rep1
GSM428129 Retina C57BL/6J control rep2
GSM428130 Retina C57BL/6J control rep3
Relations
BioProject PRJNA119831

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17117_raw.txt.gz 1.9 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap