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| Status |
Public on May 21, 2009 |
| Title |
Integration of tomato reproductive developmental landmarks and expression profiles, and the effect of SUN on fruit shape |
| Platform organism |
Solanum lycopersicum |
| Sample organism |
Solanum pimpinellifolium |
| Experiment type |
Expression profiling by array
|
| Summary |
Universally accepted landmark stages are necessary to highlight key events in tomato reproductive development. In this study, we provide a description of floral and fruit development in a red-fruited closely related wild relative of tomato, Solanum pimpinellifolium accession LA1589. We use established and propose new landmarks as the framework for the characterization of the tomato fruit shape gene SUN in fruit development. SUN controls fruit shape predominantly after fertilization and its effect reaches a maximum at 8 days post anthesis coinciding with fruit landmark 4 representing the globular embryo stage of seed development. We also analyzed gene expression profiles of floral buds 10 days before anthesis (floral landmark 7), anthesis-stage flowers (floral landmark 10 and fruit landmark 1), and 5 days post anthesis fruit (fruit landmark 3). The expression profiles of the NILs that differ at sun showed that 34 genes were differentially expressed and most of them at a less than 2-fold difference. However, many genes were differentially expressed between the developmental times points, including many genes involved in phytohormone biosynthesis or signaling as well as organ identity and patterning of tomato fruit.
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| Overall design |
Three biological replicates were conducted with three sets of LA1589 sun NILs that differ at sun growing during different time periods resulting in 3 time points x 2 genotypes x 3 replicates = 18 array hybridizations.
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| Contributor(s) |
Xiao H, van der Knaap E |
| Citation(s) |
19422692 |
| Submission date |
Mar 30, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Esther van der Knaap |
| E-mail(s) |
vanderknaap.1@osu.edu
|
| Phone |
330 263 2821
|
| Fax |
330 263 3887
|
| URL |
http://www.oardc.ohio-state.edu/vanderknaap/
|
| Organization name |
The Ohio State University
|
| Department |
Horticulture and crop science
|
| Lab |
Van der Knaap Laboratory
|
| Street address |
1680 Madison Ave.
|
| City |
Wooster |
| State/province |
OH |
| ZIP/Postal code |
44691 |
| Country |
USA |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA115951 |
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