Myh14 myosin, heavy polypeptide 14 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Myh14provided by MGI
Official Full Name: myosin, heavy polypeptide 14provided by MGI
Primary source: MGI:MGI:1919210
See related: Ensembl:ENSMUSG00000030739 AllianceGenome:MGI:1919210
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: II-C; NHMCII; NMHC II-C; 2400004E04Rik
Summary: Enables ATP binding activity; actin filament binding activity; and microfilament motor activity. Acts upstream of or within actin filament-based movement. Located in several cellular components, including brush border; growth cone; and stress fiber. Part of myosin complex. Is expressed in several structures, including alimentary system; central nervous system; respiratory system; sensory organ; and vibrissa. Human ortholog(s) of this gene implicated in autosomal dominant nonsyndromic deafness 4A and sensorineural hearing loss. Orthologous to human MYH14 (myosin heavy chain 14). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in small intestine adult (RPKM 46.6), colon adult (RPKM 41.7) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 B3; 7 28.85 cM

Exon count:: 46

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (44255227..44320296, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (44605803..44670872, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nonproductive Splicing Prevents Expression of MYH7b Protein in the Mammalian Heart.
Title: Nonproductive Splicing Prevents Expression of MYH7b Protein in the Mammalian Heart.
that Myh14 may play a beneficial role in the protection of the cochlea after acoustic overstimulation in CBA/CaJ mice
Title: Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice.
we have validated a novel gene, Myh14, as a negative regulator of ISO-induced left ventricular mass hypertrophy in an in vivo mouse model and demonstrated the up-regulation of immediate early gene Myc, fetal gene Nppb, and fibrosis gene Lgals3 in ISO-treated Myh14 deficient hearts compared to controls.
Title: Genetic Dissection of Cardiac Remodeling in an Isoproterenol-Induced Heart Failure Mouse Model.
NM II-C1C2 may be involved in stabilizing neurites by maintaining their structure at adhesion sites
Title: The effect of including the C2 insert of nonmuscle myosin II-C on neuritogenesis.
Data indicate that when both NM II-C and II-B are ablated the B-C-/B-C- cardiac myocytes show major defects in karyokinesis.
Title: Ablation of nonmuscle myosin II-B and II-C reveals a role for nonmuscle myosin II in cardiac myocyte karyokinesis.
This study provides molecular insight into the role of the structurally disordered tailpiece of non-muscle myosin IIC (NMII-C) in shifting the oligomeric equilibrium of NMII-C toward filament assembly and determining its morphology.
Title: The positively charged region of the myosin IIC non-helical tailpiece promotes filament assembly.
myosin II, directly regulates cellular patterning and alignment within the cochlear sensory epithelium
Title: Myosin II regulates extension, growth and patterning in the mammalian cochlear duct.
the alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation
Title: An alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation.
mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C
Title: Disease-associated mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C.
a cGMP-independent mechanism for nitric oxide-mediated inhibition of nonmuscle myosin heavy chain II-C induction
Title: Induction of nonmuscle myosin heavy chain II-C by butyrate in RAW 264.7 mouse macrophages.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Myh14 (e-PCR), detects polymorphism
- UniSTS:526909

RH136048 (e-PCR)
- UniSTS:210043

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to actin filament binding	ISO Inferred from Sequence Orthology more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microfilament motor activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to microfilament motor activity	ISO Inferred from Sequence Orthology more info
enables molecular adaptor activity	EXP Inferred from Experiment more info	PubMed
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin filament-based movement	IDA Inferred from Direct Assay more info	PubMed
involved_in actomyosin structure organization	ISO Inferred from Sequence Orthology more info
involved_in mitochondrion organization	ISO Inferred from Sequence Orthology more info
involved_in neuronal action potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell shape	IEA Inferred from Electronic Annotation more info
involved_in sensory perception of sound	ISO Inferred from Sequence Orthology more info
involved_in skeletal muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in skeletal muscle tissue development	ISO Inferred from Sequence Orthology more info
involved_in vocalization behavior	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in actomyosin	ISO Inferred from Sequence Orthology more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in brush border	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath	HDA more info	PubMed
part_of myosin II complex	IBA Inferred from Biological aspect of Ancestor more info
part_of myosin II complex	ISO Inferred from Sequence Orthology more info
located_in myosin II filament	ISO Inferred from Sequence Orthology more info
part_of myosin complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in myosin filament	IBA Inferred from Biological aspect of Ancestor more info
located_in stress fiber	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: myosin-14

Names: myosin heavy chain, non-muscle IIc; myosin, heavy chain 14; non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain

NP_001258467.1

EC 5.6.1.8

NP_001258469.1

EC 5.6.1.8

NP_082297.1

EC 5.6.1.8

XP_011249205.1

EC 5.6.1.8

XP_017167802.1

EC 5.6.1.8

XP_030098856.1

EC 5.6.1.8

XP_036009336.1

EC 5.6.1.8

XP_036009337.1

EC 5.6.1.8

XP_036009338.1

EC 5.6.1.8

XP_036009339.1

EC 5.6.1.8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001271538.1 → NP_001258467.1 myosin-14 isoform 1

See identical proteins and their annotated locations for NP_001258467.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC150895, AC157653

UniProtKB/TrEMBL

K3W4R2

Related

ENSMUSP00000147115.2, ENSMUST00000207775.2

Conserved Domains (3) summary

cd14930
Location:115 → 825

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam00063
Location:104 → 825

Myosin_head; Myosin head (motor domain)

pfam01576
Location:902 → 1982

Myosin_tail_1; Myosin tail
NM_001271540.1 → NP_001258469.1 myosin-14 isoform 2

See identical proteins and their annotated locations for NP_001258469.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.

Source sequence(s)

AC150895, AC157653

Consensus CDS

CCDS71951.1

UniProtKB/Swiss-Prot

B3F3T1, Q6URW6, Q80V64, Q80ZE6

UniProtKB/TrEMBL

K3W4R2

Related

ENSMUSP00000046059.8, ENSMUST00000048102.15

Conserved Domains (2) summary

cd14930
Location:115 → 792

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:869 → 1949

Myosin_tail_1; Myosin tail
NM_028021.3 → NP_082297.1 myosin-14 isoform 3

See identical proteins and their annotated locations for NP_082297.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

Source sequence(s)

AC150895, AC157653

Consensus CDS

CCDS39943.1

UniProtKB/TrEMBL

K3W4R2

Related

ENSMUSP00000103531.3, ENSMUST00000107899.10

Conserved Domains (2) summary

cd14930
Location:115 → 784

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:861 → 1941

Myosin_tail_1; Myosin tail

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

44255227..44320296 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030242996.1 → XP_030098856.1 myosin-14 isoform X2

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 784

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:861 → 1941

Myosin_tail_1; Myosin tail
XM_036153445.1 → XP_036009338.1 myosin-14 isoform X2

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 784

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:861 → 1941

Myosin_tail_1; Myosin tail
XM_036153444.1 → XP_036009337.1 myosin-14 isoform X2

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 784

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:861 → 1941

Myosin_tail_1; Myosin tail
XM_036153443.1 → XP_036009336.1 myosin-14 isoform X2

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 784

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:861 → 1941

Myosin_tail_1; Myosin tail
XM_036153446.1 → XP_036009339.1 myosin-14 isoform X2

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 784

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:861 → 1941

Myosin_tail_1; Myosin tail
XM_011250903.2 → XP_011249205.1 myosin-14 isoform X1

See identical proteins and their annotated locations for XP_011249205.1

UniProtKB/Swiss-Prot

B3F3T1, Q6URW6, Q80V64, Q80ZE6

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 792

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:869 → 1949

Myosin_tail_1; Myosin tail
XM_017312313.2 → XP_017167802.1 myosin-14 isoform X1

UniProtKB/Swiss-Prot

B3F3T1, Q6URW6, Q80V64, Q80ZE6

UniProtKB/TrEMBL

K3W4R2

Conserved Domains (2) summary

cd14930
Location:115 → 792

MYSc_Myh14_mammals; class II myosin heavy chain 14 motor domain

pfam01576
Location:869 → 1949

Myosin_tail_1; Myosin tail