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    MCM3 minichromosome maintenance complex component 3 [ Homo sapiens (human) ]

    Gene ID: 4172, updated on 5-May-2024

    Summary

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    Official Symbol
    MCM3provided by HGNC
    Official Full Name
    minichromosome maintenance complex component 3provided by HGNC
    Primary source
    HGNC:HGNC:6945
    See related
    Ensembl:ENSG00000112118 MIM:602693; AllianceGenome:HGNC:6945
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    HCC5; P1.h; RLFB; P1-MCM3
    Summary
    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
    Expression
    Ubiquitous expression in lymph node (RPKM 33.0), appendix (RPKM 23.5) and 24 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    6p12.2
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (52264015..52284742, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (52102764..52123487, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (52128813..52149540, complement)

    Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124901328 Neighboring gene solute carrier family 25 member 20 pseudogene 1 Neighboring gene interleukin 17F Neighboring gene ReSE screen-validated silencer GRCh37_chr6:52129168-52129587 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24670 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24671 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17279 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24672 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17280 Neighboring gene uncharacterized LOC124901329 Neighboring gene Sharpr-MPRA regulatory region 10632 Neighboring gene Sharpr-MPRA regulatory region 10484 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24673 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17281 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17282 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24674 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:52190539-52191144 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:52191145-52191751 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_92086 Neighboring gene Sharpr-MPRA regulatory region 5108 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:52217075-52217574 Neighboring gene ReSE screen-validated silencer GRCh37_chr6:52226353-52226538 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17283 Neighboring gene progestin and adipoQ receptor family member 8 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24676 Neighboring gene VISTA enhancer hs2064 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24678

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma. Kang EY, et al. Virchows Arch, 2022 Apr. PMID 34782936, Free PMC Article
    2. Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer. Ma H, et al. Comput Math Methods Med, 2021. PMID 34671420, Free PMC Article
    3. Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer. Zhou H, et al. Biosci Rep, 2020 Jul 31. PMID 32597491, Free PMC Article
    4. Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-ÎşB pathway. Yang Q, et al. J Exp Clin Cancer Res, 2019 Jun 17. PMID 31208444, Free PMC Article
    5. Comparison between immunohistochemical expression of Ki-67 and MCM-3 in major salivary gland epithelial tumors in children and adolescents. Preliminary study. Zieliński R, et al. Pol J Pathol, 2016. PMID 28547962

    See all (294) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma.
      Title: MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma.
    2. MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
      Title: MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
    3. Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome.
      Title: Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome.
    4. Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer.
      Title: Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer.
    5. The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids.
      Title: The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids.
    6. PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3.
      Title: PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3.
    7. MCM3 proliferative index is worthier over Ki-67 in the characterization of salivary gland tumors.
      Title: MCM3 proliferative index is worthier over Ki-67 in the characterization of salivary gland tumors.
    8. Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer.
      Title: Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer.
    9. Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients.
      Title: Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients.
    10. Results show that found DNA replication initiation protein MCM3 was upregulated in hepatocellular carcinoma (HCC) tissues and cells and its expression correlated with poor outcome and radioresistance. MCM3 promoted radioresistance through activating NF-kappaB pathway, strengthening the role of MCM subunits in the tumor progression. These Findings revealed an independent prognostic factor of MCM3 for HCC.
      Title: Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-ÎşB pathway.
    Submit: New GeneRIF Correction See all GeneRIFs (45)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Rev rev HIV-1 Rev co-localizes with minichromosome maintenance complex component 3 (MCM3) in the cytoplasmic and nucleoplasmic compartments of cells PubMed
    Tat tat HIV-1 Tat upregulates the expression of minichromosome maintenance complex component 3 (MCM3) in Jurkat cells PubMed
    tat Interaction of HIV-1 Tat with MCM3 in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry PubMed
    Vpr vpr HIV-1 Vpr downregulates MCM3 in HeLa cells within 12 hours of exposure PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC1157

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    contributes_to single-stranded DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA replication initiation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA strand elongation involved in DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA unwinding involved in DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in double-strand break repair via break-induced replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of DNA-templated DNA replication initiation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of CMG complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of CMG complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    part_of MCM complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of MCM complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of MCM complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of alpha DNA polymerase:primase complex TAS
    Traceable Author Statement
    more info
    		 PubMed 
    located_in centrosome IDA
    Inferred from Direct Assay
    more info
    		  
    located_in chromosome, telomeric region HDA PubMed 
    located_in membrane HDA PubMed 
    NOT located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA replication licensing factor MCM3
    Names
    DNA polymerase alpha holoenzyme-associated protein P1
    DNA replication factor MCM3
    MCM3 minichromosome maintenance deficient 3
    RLF subunit beta
    cervical cancer proto-oncogene 5
    hRlf beta subunit
    minichromosome maintenance deficient 3
    p102
    replication licensing factor, beta subunit
    NP_001257401.2
    NP_001353298.1
    NP_001353299.1
    NP_001353300.1
    NP_001353301.1
    NP_001353302.1
    NP_001353303.1
    NP_001353304.1
    NP_002379.4

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001270472.3 → NP_001257401.2  DNA replication licensing factor MCM3 isoform 2

      Status: REVIEWED

      Source sequence(s)
      AL034343
      Consensus CDS
      CCDS75468.2
      UniProtKB/TrEMBL
      B4DUQ9, J3KQ69
      Related
      ENSP00000229854.6, ENST00000229854.12
      Conserved Domains (1) summary
      smart00350
      Location:63 → 608
      MCM; minichromosome maintenance proteins

    2. NM_001366369.2 → NP_001353298.1  DNA replication licensing factor MCM3 isoform 3

      Status: REVIEWED

      Source sequence(s)
      AL034343
      UniProtKB/TrEMBL
      A0A0S2Z492, Q53HJ4
      Conserved Domains (2) summary
      smart00350
      Location:109 → 654
      MCM; minichromosome maintenance proteins
      pfam14551
      Location:19 → 104
      MCM_N; MCM N-terminal domain

    3. NM_001366370.2 → NP_001353299.1  DNA replication licensing factor MCM3 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AL034343
      UniProtKB/TrEMBL
      Q53HJ4
      Conserved Domains (2) summary
      smart00350
      Location:109 → 671
      MCM; minichromosome maintenance proteins
      pfam14551
      Location:19 → 104
      MCM_N; MCM N-terminal domain

    4. NM_001366371.2 → NP_001353300.1  DNA replication licensing factor MCM3 isoform 5

      Status: REVIEWED

      Source sequence(s)
      AL034343
      UniProtKB/TrEMBL
      Q53HJ4
      Conserved Domains (2) summary
      smart00350
      Location:109 → 637
      MCM; minichromosome maintenance proteins
      pfam14551
      Location:19 → 104
      MCM_N; MCM N-terminal domain

    5. NM_001366372.2 → NP_001353301.1  DNA replication licensing factor MCM3 isoform 6

      Status: REVIEWED

      Source sequence(s)
      AL034343
      UniProtKB/TrEMBL
      Q53HJ4
      Conserved Domains (2) summary
      smart00350
      Location:109 → 637
      MCM; minichromosome maintenance proteins
      pfam14551
      Location:19 → 104
      MCM_N; MCM N-terminal domain

    6. NM_001366373.2 → NP_001353302.1  DNA replication licensing factor MCM3 isoform 7

      Status: REVIEWED

      Source sequence(s)
      AL034343
      UniProtKB/TrEMBL
      B4DS46
      Conserved Domains (1) summary
      cl25443
      Location:6 → 481
      MCM_OB; MCM OB domain

    7. NM_001366374.2 → NP_001353303.1  DNA replication licensing factor MCM3 isoform 8

      Status: REVIEWED

      Source sequence(s)
      AL034343
      UniProtKB/TrEMBL
      B4DS46
      Conserved Domains (1) summary
      cl25443
      Location:1 → 451
      MCM_OB; MCM OB domain

    8. NM_001366375.2 → NP_001353304.1  DNA replication licensing factor MCM3 isoform 9

      Status: REVIEWED

      Source sequence(s)
      AL034343
      Conserved Domains (2) summary
      COG1241
      Location:17 → 165
      Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
      cl25443
      Location:177 → 373
      MCM_OB; MCM OB domain

    9. NM_002388.6 → NP_002379.4  DNA replication licensing factor MCM3 isoform 1

      Status: REVIEWED

      Source sequence(s)
      AL034343
      Consensus CDS
      CCDS4940.3
      UniProtKB/Swiss-Prot
      B4DWW4, P25205, Q92660, Q9BTR3, Q9NUE7
      UniProtKB/TrEMBL
      A0A0S2Z4T1, Q53HJ4
      Related
      ENSP00000472940.2, ENST00000596288.7
      Conserved Domains (2) summary
      smart00350
      Location:109 → 654
      MCM; minichromosome maintenance proteins
      pfam14551
      Location:16 → 102
      MCM_N; MCM N-terminal domain

    RNA

    1. NR_158963.2 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AL034343

    2. NR_158964.2 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AL034343

    3. NR_158965.2 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AL034343

    4. NR_158966.2 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AL034343

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

      Range
      52264015..52284742 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060930.1 Alternate T2T-CHM13v2.0

      Range
      52102764..52123487 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25205.3 GenPept UniProtKB/Swiss-Prot:P25205

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