Dgkq diacylglycerol kinase, theta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkqprovided by MGI
Official Full Name: diacylglycerol kinase, thetaprovided by MGI
Primary source: MGI:MGI:102918
See related: Ensembl:ENSMUSG00000004815 AllianceGenome:MGI:102918
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DAGK; Dgkd; DAGK7; Dagk4; 110kDa
Summary: Predicted to enable several functions, including diacylglycerol kinase activity; enzyme binding activity; and transmembrane receptor protein tyrosine kinase activator activity. Involved in regulation of synaptic vesicle endocytosis. Located in glutamatergic synapse and presynapse. Is active in postsynapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Orthologous to human DGKQ (diacylglycerol kinase theta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in small intestine adult (RPKM 17.4), duodenum adult (RPKM 15.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 53.24 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (108794910..108808696, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (108647044..108660830, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Roles of DGKs in neurons: Postsynaptic functions?
Title: Roles of DGKs in neurons: Postsynaptic functions?
Results suggest that DGKtheta; may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages.
Title: The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos.
Diacylglycerol kinase theta is regulated by magnesium and zinc.
Title: Diacylglycerol kinase θ: regulation and stability.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dgkq (e-PCR), detects polymorphism
- UniSTS:546735

AI504381 (e-PCR)
- UniSTS:159411

NoName (e-PCR)
- UniSTS:235249

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables alkylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phospholipase binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables transmembrane receptor protein tyrosine kinase activator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cAMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein kinase C signaling	ISO Inferred from Sequence Orthology more info
involved_in protein kinase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in regulation of G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in regulation of cholesterol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of cortisol biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in regulation of progesterone biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in response to ATP	ISO Inferred from Sequence Orthology more info
involved_in response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in thrombin-activated receptor signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endosome	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	IEP Inferred from Expression Pattern more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info
located_in vesicle membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: diacylglycerol kinase theta

Names: DAG kinase theta; DGK-theta; Dgk theta; diacylglycerol kinase, delta (110 kDa); diglyceride kinase theta

NP_001343958.1

EC 2.7.1.107

EC 2.7.1.93

NP_950176.1

EC 2.7.1.107

EC 2.7.1.93

XP_006534789.1

EC 2.7.1.107

EC 2.7.1.93

XP_011247694.1

EC 2.7.1.107

EC 2.7.1.93

XP_030109915.1

EC 2.7.1.107

EC 2.7.1.93

XP_030109916.1

EC 2.7.1.107

EC 2.7.1.93

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357029.1 → NP_001343958.1 diacylglycerol kinase theta isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice junction compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AC161813

Conserved Domains (4) summary

smart00045
Location:498 → 650

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:347 → 471

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd01783
Location:154 → 248

RA2_DAGK-theta; Ras-associating (RA) domain 2 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins

cd17111
Location:58 → 151

RA1_DAGK-theta; Ras-associating (RA) domain 1 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins
NM_199011.2 → NP_950176.1 diacylglycerol kinase theta isoform 1

See identical proteins and their annotated locations for NP_950176.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC161813, BC062929

Consensus CDS

CCDS19515.1

UniProtKB/Swiss-Prot

Q3UYE8, Q6P5E8

Related

ENSMUSP00000057859.7, ENSMUST00000053913.13

Conserved Domains (6) summary

TIGR00147
Location:582 → 904

TIGR00147; lipid kinase, YegS/Rv2252/BmrU family

smart00109
Location:178 → 228

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:733 → 885

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:582 → 706

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:55 → 102

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl00155
Location:388 → 483

UBQ; Ubiquitin-like proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

108794910..108808696 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011249392.2 → XP_011247694.1 diacylglycerol kinase theta isoform X1

Conserved Domains (6) summary

smart00109
Location:178 → 228

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:738 → 890

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:587 → 711

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:55 → 102

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01783
Location:389 → 483

RA2_DAGK-theta; Ras-associating (RA) domain 2 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins

cd17111
Location:293 → 386

RA1_DAGK-theta; Ras-associating (RA) domain 1 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins
XM_030254055.2 → XP_030109915.1 diacylglycerol kinase theta isoform X2

Conserved Domains (4) summary

smart00045
Location:503 → 655

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:352 → 476

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd01783
Location:154 → 248

RA2_DAGK-theta; Ras-associating (RA) domain 2 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins

cd17111
Location:58 → 151

RA1_DAGK-theta; Ras-associating (RA) domain 1 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins
XM_006534726.4 → XP_006534789.1 diacylglycerol kinase theta isoform X3

Conserved Domains (4) summary

smart00109
Location:178 → 228

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

cd00029
Location:55 → 102

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01783
Location:389 → 483

RA2_DAGK-theta; Ras-associating (RA) domain 2 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins

cd17111
Location:293 → 386

RA1_DAGK-theta; Ras-associating (RA) domain 1 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins
XM_030254056.2 → XP_030109916.1 diacylglycerol kinase theta isoform X4

Conserved Domains (5) summary

smart00109
Location:178 → 228

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

cd00029
Location:55 → 102

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd17111
Location:293 → 386

RA1_DAGK-theta; Ras-associating (RA) domain 1 found in diacylgylcerol kinase theta (DAGK-theta) and similar proteins

cl00040
Location:125 → 162

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl28922
Location:389 → 474

Ubiquitin_like_fold; Beta-grasp ubiquitin-like fold