MARK2 microtubule affinity regulating kinase 2 [Homo sapiens (human)] - Gene

Summary

Official Symbol: MARK2provided by HGNC
Official Full Name: microtubule affinity regulating kinase 2provided by HGNC
Primary source: HGNC:HGNC:3332
See related: Ensembl:ENSG00000072518 MIM:600526; AllianceGenome:HGNC:3332
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: EMK1; EMK-1; PAR-1; Par1b; Par-1b
Summary: This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
Expression: Ubiquitous expression in esophagus (RPKM 11.9), colon (RPKM 10.6) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 11q13.1

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	11	NC_000011.10 (63839110..63911020)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	11	NC_060935.1 (63828449..63900374)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	11	NC_000011.9 (63606582..63678492)

Chromosome 11 - NC_000011.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize vesicular transport of transferrin receptor within dendrites.
Title: MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize vesicular transport of transferrin receptor within dendrites.
Identification of a reciprocal negative feedback loop between tau-modifying proteins MARK2 kinase and CBP acetyltransferase.
Title: Identification of a reciprocal negative feedback loop between tau-modifying proteins MARK2 kinase and CBP acetyltransferase.
MARK2 potentiate aerobic glycolysis-mediated cell growth in breast cancer through regulating mTOR/HIF-1alpha and p53 pathways.
Title: MARK2 potentiate aerobic glycolysis-mediated cell growth in breast cancer through regulating mTOR/HIF-1α and p53 pathways.
MARK2/4 promotes Warburg effect and cell growth in non-small cell lung carcinoma through the AMPKalpha1/mTOR/HIF-1alpha signaling pathway.
Title: MARK2/4 promotes Warburg effect and cell growth in non-small cell lung carcinoma through the AMPKα1/mTOR/HIF-1α signaling pathway.
Long non-coding RNA ABHD11-AS1 facilitates the progression of cervical cancer by competitively binding to miR-330-5p and upregulating MARK2.
Title: Long non-coding RNA ABHD11-AS1 facilitates the progression of cervical cancer by competitively binding to miR-330-5p and upregulating MARK2.
The cell polarity kinase Par1b/MARK2 activation selects specific NF-kB transcripts via phosphorylation of core mediator Med17/TRAP80.
Title: The cell polarity kinase Par1b/MARK2 activation selects specific NF-kB transcripts via phosphorylation of core mediator Med17/TRAP80.
MARK2 phosphorylates eIF2alpha in response to proteotoxic stress.
Title: MARK2 phosphorylates eIF2α in response to proteotoxic stress.
Study shows that MARK2 is required to recentre spindles that are off-centred following actin disassembly, showing the close functional relationship between MARK2 and the actin network. These results suggest that, during both interphase and mitosis, MARK2 localizes at specialized membrane subdomains and coordinates actin and microtubule cytoskeletal changes, thus enabling normal cell division.
Title: MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.
Par1b-inhibition by CagA contributes to DNA Double Strand Breaks in H. pylori infected human primary gastric epithelial cells.
Title: Inhibition of polarity-regulating kinase PAR1b contributes to Helicobacter pylori inflicted DNA Double Strand Breaks in gastric cells.
Clustering of the CD44 extracellular domain by high-molecular-weight hyaluronan leads to recruitment of the polarity-regulating kinase PAR1b by the CD44 intracellular domain, which results in disruption of the Hippo signaling-inhibitory PAR1b-MST complex.
Title: High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.

Submit: New GeneRIF Correction See all GeneRIFs (43)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of blood pressure response to methylphenidate treatment of attention-deficit/hyperactivity disorder. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11S1227 (e-PCR)
- UniSTS:36420

RH47044 (e-PCR)
- UniSTS:11853

D11S1226 (e-PCR)
- UniSTS:38779

MARC_7553-7554:992008491:1 (e-PCR)
- UniSTS:231343

MARC_7553-7554:996687939:1 (e-PCR)
- UniSTS:231343

D11S993 (e-PCR)
- UniSTS:90295

STS-R39857 (e-PCR)
- UniSTS:55800

D11S3862 (e-PCR)
- UniSTS:153085

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	HDA more info	PubMed
enables cadherin binding	HDA more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activator activity	TAS Traceable Author Statement more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein serine/threonine kinase activity	NAS Non-traceable Author Statement more info	PubMed
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed
enables tau protein binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables tau protein binding	NAS Non-traceable Author Statement more info	PubMed
enables tau-protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables tau-protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables tau-protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables tau-protein kinase activity	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in activation of protein kinase activity	TAS Traceable Author Statement more info	PubMed
involved_in autophagy of mitochondrion	NAS Non-traceable Author Statement more info	PubMed
involved_in axon development	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in establishment of cell polarity	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of cell polarity	TAS Traceable Author Statement more info	PubMed
involved_in establishment or maintenance of cell polarity regulating cell shape	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion localization	NAS Non-traceable Author Statement more info	PubMed
involved_in neuron migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in peptidyl-threonine phosphorylation	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of neuron projection development	IDA Inferred from Direct Assay more info	PubMed
involved_in protein autophosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of microtubule binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in regulation of microtubule cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in lateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in microtubule bundle	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	NAS Non-traceable Author Statement more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine/threonine-protein kinase MARK2

Names: ELKL motif kinase 1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog b; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; testicular tissue protein Li 117

NP_001034558.2

EC 2.7.11.1

EC 2.7.11.26

NP_001156768.1

EC 2.7.11.1

EC 2.7.11.26

NP_001156769.1

EC 2.7.11.1

EC 2.7.11.26

NP_004945.4

EC 2.7.11.1

EC 2.7.11.26

NP_059672.2

EC 2.7.11.1

EC 2.7.11.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029771.1 RefSeqGene

Range

5183..77093

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001039469.3 → NP_001034558.2 serine/threonine-protein kinase MARK2 isoform d

See identical proteins and their annotated locations for NP_001034558.2

Status: REVIEWED

Description

Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (d).

Source sequence(s)

AB188493, AK225766, AP003780, BC008771, BM719402

Consensus CDS

CCDS53649.1

UniProtKB/Swiss-Prot

Q15449, Q15524, Q5XGA3, Q68A18, Q7KZI7, Q96HB3, Q96RG0

UniProtKB/TrEMBL

A8K2S4

Related

ENSP00000385751.2, ENST00000402010.8

Conserved Domains (3) summary

cd12201
Location:689 → 787

MARK2_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 2

cd14406
Location:322 → 363

UBA_MARK2; UBA domain found in serine/threonine-protein kinase MARK2 and similar proteins

cd14072
Location:52 → 304

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases
NM_001163296.2 → NP_001156768.1 serine/threonine-protein kinase MARK2 isoform e

See identical proteins and their annotated locations for NP_001156768.1

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks two alternate in-frame exons in the 3' coding region, compared to variant 4. The resulting isoform (e) lacks two internal segments, compared to isoform d.

Source sequence(s)

AB188493, AK225766, AP003780, BM719402

Consensus CDS

CCDS53650.1

UniProtKB/TrEMBL

A0A0A0MRU9, A0A140VJP1

Related

ENSP00000355091.5, ENST00000361128.9

Conserved Domains (3) summary

cd12201
Location:620 → 718

MARK2_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 2

cd14406
Location:322 → 363

UBA_MARK2; UBA domain found in serine/threonine-protein kinase MARK2 and similar proteins

cd14072
Location:52 → 304

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases
NM_001163297.2 → NP_001156769.1 serine/threonine-protein kinase MARK2 isoform f

See identical proteins and their annotated locations for NP_001156769.1

Status: REVIEWED

Description

Transcript Variant: This variant (6) lacks three alternate in-frame exons and uses an alternate in-frame splice site, compared to variant 4. The resulting isoform (f) lacks two internal segments and 1 internal residue, compared to isoform d.

Source sequence(s)

AK225766, AP003780, BM719402

Consensus CDS

CCDS53651.1

UniProtKB/TrEMBL

A0A0A0MRU9

Related

ENSP00000294247.9, ENST00000350490.11

Conserved Domains (3) summary

cd12201
Location:610 → 708

MARK2_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 2

cd14406
Location:322 → 363

UBA_MARK2; UBA domain found in serine/threonine-protein kinase MARK2 and similar proteins

cd14072
Location:52 → 304

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases
NM_004954.5 → NP_004945.4 serine/threonine-protein kinase MARK2 isoform c

See identical proteins and their annotated locations for NP_004945.4

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region and uses an alternate in-frame splice site in the central coding region, compared to variant 4. The resulting isoform (c) lacks two internal segments and 1 internal residue, compared to isoform d.

Source sequence(s)

AK225766, AP003780, BC084540, BM719402

Consensus CDS

CCDS8051.2

UniProtKB/TrEMBL

A0A0A0MRU9

Related

ENSP00000425765.1, ENST00000508192.5

Conserved Domains (3) summary

cd12201
Location:625 → 723

MARK2_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 2

cd14406
Location:322 → 363

UBA_MARK2; UBA domain found in serine/threonine-protein kinase MARK2 and similar proteins

cd14072
Location:52 → 304

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases
NM_017490.4 → NP_059672.2 serine/threonine-protein kinase MARK2 isoform a

See identical proteins and their annotated locations for NP_059672.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, lacks an in-frame exon, and uses an alternate in-frame splice site, compared to variant 4. The resulting isoform (a) has a shorter N-terminus and lacks an internal segment and 1 internal residue, compared to isoform d.

Source sequence(s)

AK290339, AP003780, BM719402, BT007342, DA855402

Consensus CDS

CCDS41665.1

UniProtKB/TrEMBL

A8K2S4

Related

ENSP00000423974.2, ENST00000509502.6

Conserved Domains (3) summary

cd12201
Location:646 → 744

MARK2_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 2

cd14406
Location:289 → 330

UBA_MARK2; UBA domain found in serine/threonine-protein kinase MARK2 and similar proteins

cd14072
Location:19 → 271

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases