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    Spaca6 sperm acrosome associated 6 [ Mus musculus (house mouse) ]

    Gene ID: 75202, updated on 9-May-2024

    Summary

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    Official Symbol
    Spaca6provided by MGI
    Official Full Name
    sperm acrosome associated 6provided by MGI
    Primary source
    MGI:MGI:1922452
    See related
    Ensembl:ENSMUSG00000080316 AllianceGenome:MGI:1922452
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    let-7e; SPACA6A; mir-99b; mir-125a; Ncrna00085; 4930546H06Rik; 9530079D04Rik; B230206P06Rik
    Summary
    Involved in fusion of sperm to egg plasma membrane involved in single fertilization. Located in acrosomal vesicle. Orthologous to human SPACA6 (sperm acrosome associated 6). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in CNS E11.5 (RPKM 6.5), limb E14.5 (RPKM 5.9) and 24 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Spaca6 in Genome Data Viewer
    Location:
    17 A3.2; 17 10.5 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (18045955..18063274)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (17826085..17843010)

    Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene farnesyl diphosphate synthetase pseudogene Neighboring gene vomeronasal 2, receptor 90 Neighboring gene STARR-seq mESC enhancer starr_41964 Neighboring gene STARR-seq mESC enhancer starr_41965 Neighboring gene STARR-seq mESC enhancer starr_41966 Neighboring gene STARR-seq mESC enhancer starr_41967 Neighboring gene STARR-seq mESC enhancer starr_41968 Neighboring gene microRNA let7e Neighboring gene microRNA 125a Neighboring gene microRNA 99b Neighboring gene predicted gene, 36907 Neighboring gene hyaluronan synthase 1 Neighboring gene formyl peptide receptor 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function. Bhatlekar S, et al. Blood Cells Mol Dis, 2021 Dec. PMID 34775219, Free PMC Article
    2. Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion. Barbaux S, et al. Sci Rep, 2020 Mar 24. PMID 32210282, Free PMC Article
    3. Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments. Grossman H, et al. Sci Rep, 2017 May 22. PMID 28533542, Free PMC Article
    4. miR-125a-5p impairs endothelial cell angiogenesis in aging mice via RTEF-1 downregulation. Che P, et al. Aging Cell, 2014 Oct. PMID 25059272, Free PMC Article
    5. MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells. Xu Y, et al. J Agric Food Chem, 2021 Mar 3. PMID 33599498

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Regulation of Zbp1 by miR-99b-5p in microglia controls the development of schizophrenia-like symptoms in mice.
      Title: Regulation of Zbp1 by miR-99b-5p in microglia controls the development of schizophrenia-like symptoms in mice.
    2. Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function.
      Title: Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function.
    3. MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells.
      Title: MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells.
    4. Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion.
      Title: Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion.
    5. Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
      Title: Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
    6. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.
      Title: Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments.
    7. Dual-luciferase reporter gene analysis showed that RTEF-1 is a direct target of mir-125a-5p, which regulates angiogenesis by repressing RTEF-1 expression and modulating eNOS and VEGF expression.
      Title: miR-125a-5p impairs endothelial cell angiogenesis in aging mice via RTEF-1 downregulation.
    8. The Spaca6 gene product may, together with Izumo1, mediate sperm fusion by binding an as yet unidentified egg membrane receptor.
      Title: A transgenic insertion on mouse chromosome 17 inactivates a novel immunoglobulin superfamily gene potentially involved in sperm-egg fusion.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables molecular_function ND
    No biological Data available
    more info
    		  
    Process Evidence Code Pubs
    involved_in fusion of sperm to egg plasma membrane involved in single fertilization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within fusion of sperm to egg plasma membrane involved in single fertilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in fusion of sperm to egg plasma membrane involved in single fertilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within single fertilization IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in acrosomal vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasmic vesicle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sperm acrosome membrane-associated protein 6
    Names
    immunoglobulin domain-containing protein

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001162909.1 → NP_001156381.1  sperm acrosome membrane-associated protein 6 precursor

      Status: PROVISIONAL

      Source sequence(s)
      AC165361
      Consensus CDS
      CCDS49967.1
      UniProtKB/Swiss-Prot
      B7ZWC7, D3Z1S3, E9Q8Q8, J3QMI7
      Related
      ENSMUSP00000128732.3, ENSMUST00000172097.9
      Conserved Domains (2) summary
      smart00410
      Location:172 → 242
      IG_like; Immunoglobulin like
      cl11960
      Location:175 → 245
      Ig; Immunoglobulin domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000083.7 Reference GRCm39 C57BL/6J

      Range
      18045955..18063274
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011246692.4 → XP_011244994.1  sperm acrosome membrane-associated protein 6 isoform X7

    2. XM_017317705.3 → XP_017173194.1  sperm acrosome membrane-associated protein 6 isoform X9

      Conserved Domains (1) summary
      pfam12101
      Location:58 → 78
      DUF3577; Protein of unknown function (DUF3577)

    3. XM_006525053.4 → XP_006525116.1  sperm acrosome membrane-associated protein 6 isoform X1

    4. XM_036160818.1 → XP_036016711.1  sperm acrosome membrane-associated protein 6 isoform X2

    5. XM_006525055.5 → XP_006525118.1  sperm acrosome membrane-associated protein 6 isoform X3

      Conserved Domains (1) summary
      cl09930
      Location:12 → 70
      RPA_2b-aaRSs_OBF_like; Replication protein A, class 2b aminoacyl-tRNA synthetases, and related proteins with oligonucleotide/oligosaccharide (OB) fold.

    6. XM_036160819.1 → XP_036016712.1  sperm acrosome membrane-associated protein 6 isoform X5

    7. XM_006525056.5 → XP_006525119.1  sperm acrosome membrane-associated protein 6 isoform X4

    8. XM_030250121.2 → XP_030105981.1  sperm acrosome membrane-associated protein 6 isoform X6

    9. XM_011246695.4 → XP_011244997.1  sperm acrosome membrane-associated protein 6 isoform X8

      See identical proteins and their annotated locations for XP_011244997.1

      Conserved Domains (1) summary
      pfam12101
      Location:58 → 78
      DUF3577; Protein of unknown function (DUF3577)

    RNA

    1. XR_385379.5 RNA Sequence

    2. XR_876567.4 RNA Sequence

    3. XR_876568.4 RNA Sequence

    4. XR_876572.4 RNA Sequence

    5. XR_003952208.2 RNA Sequence

    6. XR_385377.4 RNA Sequence

    7. XR_003952205.2 RNA Sequence

    8. XR_876569.4 RNA Sequence

    9. XR_385378.5 RNA Sequence

    10. XR_004939594.1 RNA Sequence

    11. XR_003952206.2 RNA Sequence

    12. XR_004939595.1 RNA Sequence

    13. XR_004939596.1 RNA Sequence

    14. XR_004939593.1 RNA Sequence

    15. XR_003952207.2 RNA Sequence

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_029205.2: Suppressed sequence

      Description
      NM_029205.2: This RefSeq was permanently suppressed because currently there is support for the transcript but not for the protein.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    E9Q8Q8.1 GenPept UniProtKB/Swiss-Prot:E9Q8Q8

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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