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    Mpg N-methylpurine-DNA glycosylase [ Mus musculus (house mouse) ]

    Gene ID: 268395, updated on 9-May-2024

    Summary

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    Official Symbol
    Mpgprovided by MGI
    Official Full Name
    N-methylpurine-DNA glycosylaseprovided by MGI
    Primary source
    MGI:MGI:97073
    See related
    Ensembl:ENSMUSG00000020287 AllianceGenome:MGI:97073
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Aag; APNG; Mid1; 9830006D05
    Summary
    Enables alkylbase DNA N-glycosylase activity. Acts upstream of or within base-excision repair. Predicted to be located in cytosol and nucleoplasm. Is expressed in several structures, including early conceptus; gonad; hemolymphoid system gland; musculature; and placenta. Orthologous to human MPG (N-methylpurine DNA glycosylase). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in adrenal adult (RPKM 8.1), genital fat pad adult (RPKM 7.4) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    11 A4; 11 18.83 cM
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (32176505..32182702)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (32226505..32232702)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E730 Neighboring gene small nuclear ribonucleoprotein 25 (U11/U12) Neighboring gene rhomboid 5 homolog 1 Neighboring gene STARR-positive B cell enhancer ABC_E11473 Neighboring gene alpha-globin locus regulatory region Neighboring gene nitrogen permease regulator-like 3 Neighboring gene hemoglobin X, alpha-like embryonic chain in Hba complex Neighboring gene hemoglobin alpha, adult chain 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Kiraly O, et al. Carcinogenesis, 2014 Nov. PMID 25155011, Free PMC Article
    2. DNA breaks and chromosomal aberrations arise when replication meets base excision repair. Ensminger M, et al. J Cell Biol, 2014 Jul 7. PMID 24982429, Free PMC Article
    3. N-terminal extension of N-methylpurine DNA glycosylase is required for turnover in hypoxanthine excision reaction. Adhikari S, et al. J Biol Chem, 2007 Oct 12. PMID 17716976
    4. The effect of dietary folic acid deficiency on the cytotoxic and mutagenic responses to methyl methanesulfonate in wild-type and in 3-methyladenine DNA glycosylase-deficient Aag null mice. Branda RF, et al. Mutat Res, 2007 Feb 3. PMID 17207504
    5. Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate. Barbin A, et al. Cancer Res, 2003 Nov 15. PMID 14633693

    See all (60) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Alkyladenine DNA glycosylase deficiency uncouples alkylation-induced strand break generation from PARP-1 activation and glycolysis inhibition.
      Title: Alkyladenine DNA glycosylase deficiency uncouples alkylation-induced strand break generation from PARP-1 activation and glycolysis inhibition.
    2. ZEB1 promotes colitis and inflammatory CRC through the inhibition of MPG in epithelial cells, thus offering new therapeutic strategies to modulate inflammation and inflammatory cancer
      Title: ZEB1 promotes inflammation and progression towards inflammation-driven carcinoma through repression of the DNA repair glycosylase MPG in epithelial cells.
    3. The depletion of Aag did not significantly change the transcriptional inhibitory or mutagenic properties of all five examined lesions, suggesting a negligible role of Aag in the repair of these DNA adducts in mammalian cells.
      Title: Roles of Aag, Alkbh2, and Alkbh3 in the Repair of Carboxymethylated and Ethylated Thymidine Lesions.
    4. This study demonstrates for the first time a non-linear dose-response for alkylation-induced colorectal carcinogenesis and reveals DNA repair by MGMT, but not AAG, as a key node in determining a carcinogenic threshold.
      Title: DNA repair by MGMT, but not AAG, causes a threshold in alkylation-induced colorectal carcinogenesis.
    5. the detrimental effects of Aag-initiated BER during I/R and sterile inflammation, and present a novel target for controlling I/R-induced injury.
      Title: Aag-initiated base excision repair promotes ischemia reperfusion injury in liver, brain, and kidney.
    6. Toxicity, induced by tert-butyl-hydroperoxide and potassium bromate, differs in base excision repair proficient (Mpg (+/+), Nth1 (+/+)) and deficient (Mpg (-/-), Nth1 (-/-)) mouse embryonic fibroblasts following Msh2 knockdown, was examined.
      Title: The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.
    7. These results provide in vivo evidence that Aag-initiated BER may play a critical role in determining the side-effects of alkylating agent chemotherapies and that Parp1 plays a crucial role in Aag-mediated tissue damage.
      Title: Aag DNA glycosylase promotes alkylation-induced tissue damage mediated by Parp1.
    8. ALKBH2 and ALKBH3 provide cancer protection similar to that of the DNA glycosylase AAG and display apparent epistasis with Aag
      Title: DNA repair is indispensable for survival after acute inflammation.
    9. RNS-induced posttranslational modification of AAG is one mechanism of base excision repair dysregulation
      Title: Differential effects of reactive nitrogen species on DNA base excision repair initiated by the alkyladenine DNA glycosylase.
    10. Clock gene mutation modulates the cellular sensitivity to genotoxic stress through altering the expression of Mpg gene.
      Title: Clock gene mutation modulates the cellular sensitivity to genotoxic stress through altering the expression of N-methylpurine DNA glycosylase gene.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-3-methyladenine glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-3-methylguanine glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-7-methyladenine glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-7-methylguanine glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables alkylbase DNA N-glycosylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables alkylbase DNA N-glycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables alkylbase DNA N-glycosylase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within base-excision repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial nucleoid IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DNA-3-methyladenine glycosylase
    Names
    3-alkyladenine DNA glycosylase
    3-methyladenine DNA glycosidase
    3-methyladenine DNA glycosylase
    ADPG
    alkylpurine-DNA-N-glycosylase
    NP_034952.2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_010822.4 → NP_034952.2  DNA-3-methyladenine glycosylase

      See identical proteins and their annotated locations for NP_034952.2

      Status: VALIDATED

      Source sequence(s)
      AL929446
      Consensus CDS
      CCDS24520.1
      UniProtKB/Swiss-Prot
      A2AVE7, Q04841, Q64182
      UniProtKB/TrEMBL
      Q3U1C9
      Related
      ENSMUSP00000020528.8, ENSMUST00000020528.14
      Conserved Domains (1) summary
      cd00540
      Location:110 → 307
      AAG; Alkyladenine DNA glycosylase catalyzes the first step in base excision repair

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      32176505..32182702
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q04841.3 GenPept UniProtKB/Swiss-Prot:Q04841

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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