Pkn3 protein kinase N3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pkn3provided by MGI
Official Full Name: protein kinase N3provided by MGI
Primary source: MGI:MGI:2388285
See related: Ensembl:ENSMUSG00000026785 AllianceGenome:MGI:2388285
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in epithelial cell migration; intracellular signal transduction; and peptidyl-serine phosphorylation. Predicted to act upstream of or within phosphorylation. Predicted to be located in cytoplasm and nucleus. Is expressed in cardiovascular system; genitourinary system; respiratory system; spleen; and submandibular gland. Orthologous to human PKN3 (protein kinase N3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in lung adult (RPKM 8.5), subcutaneous fat pad adult (RPKM 6.3) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 B; 2 21.13 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (29967657..29981031)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (30077548..30091023)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

a novel interaction of p130Cas with Ser/Thr kinase PKN3, is reported.
Title: The interaction of p130Cas with PKN3 promotes malignant growth.
Pkn3 promotes bone resorption downstream of Wnt5a-Ror2-Rho signaling, and this pathway may be a therapeutic target for bone diseases such as osteoporosis, rheumatoid arthritis, and periodontal disease.
Title: Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling.
PKN3 is the major regulator of angiogenesis in humans and mice and tumor metastasis in mice.
Title: PKN3 is the major regulator of angiogenesis and tumor metastasis in mice.
Data demonstrate that PKN3 physically interacts with Rho-family GTPases, and preferentially with RhoC, a known mediator of tumor invasion and metastasis in epithelial cancers.
Title: The interaction of PKN3 with RhoC promotes malignant growth.
PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of phosphoinositide 3-kinase
Title: PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in epithelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase N3

Names: protein kinase PKN-beta; protein kinase PKNbeta; protein-kinase C-related kinase 3

NP_722500.1

EC 2.7.11.13

XP_006498115.1

EC 2.7.11.13

XP_006498116.1

EC 2.7.11.13

XP_006498117.1

EC 2.7.11.13

XP_030107417.1

EC 2.7.11.13

XP_030107418.1

EC 2.7.11.13

XP_030107419.1

EC 2.7.11.13

XP_030107420.1

EC 2.7.11.13

XP_030107421.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153805.2 → NP_722500.1 serine/threonine-protein kinase N3

See identical proteins and their annotated locations for NP_722500.1

Status: VALIDATED

Source sequence(s)

BX005298

Consensus CDS

CCDS15867.1

UniProtKB/Swiss-Prot

Q8K045

Related

ENSMUSP00000041025.8, ENSMUST00000045246.8

Conserved Domains (5) summary

PTZ00263
Location:544 → 868

PTZ00263; protein kinase A catalytic subunit; Provisional

cd11622
Location:10 → 75

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:548 → 873

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:90 → 163

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:321 → 408

C2; C2 domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

29967657..29981031

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030251558.2 → XP_030107418.1 serine/threonine-protein kinase N3 isoform X2

Conserved Domains (4) summary

cd11622
Location:14 → 79

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:575 → 900

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:94 → 167

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:348 → 435

C2; C2 domain
XM_006498053.5 → XP_006498116.1 serine/threonine-protein kinase N3 isoform X6

See identical proteins and their annotated locations for XP_006498116.1

Conserved Domains (4) summary

cd11622
Location:14 → 79

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:552 → 877

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:94 → 167

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:325 → 412

C2; C2 domain
XM_030251559.2 → XP_030107419.1 serine/threonine-protein kinase N3 isoform X3

Conserved Domains (4) summary

cd11622
Location:13 → 78

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:574 → 899

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:93 → 166

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:347 → 434

C2; C2 domain
XM_030251561.2 → XP_030107421.1 serine/threonine-protein kinase N3 isoform X8

Conserved Domains (5) summary

cd11622
Location:23 → 88

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

PRK14950
Location:477 → 579

PRK14950; DNA polymerase III subunits gamma and tau; Provisional

cl00087
Location:103 → 176

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:357 → 444

C2; C2 domain

cl21453
Location:584 → 600

PKc_like; Protein Kinases, catalytic domain
XM_030251557.2 → XP_030107417.1 serine/threonine-protein kinase N3 isoform X1

Conserved Domains (4) summary

cd11622
Location:23 → 88

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:584 → 909

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:103 → 176

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:357 → 444

C2; C2 domain
XM_006498052.5 → XP_006498115.1 serine/threonine-protein kinase N3 isoform X5

Conserved Domains (4) summary

cd11622
Location:23 → 88

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:561 → 886

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:103 → 176

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:334 → 421

C2; C2 domain
XM_030251560.2 → XP_030107420.1 serine/threonine-protein kinase N3 isoform X4

Conserved Domains (4) summary

cd11622
Location:10 → 75

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd05589
Location:571 → 896

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:90 → 163

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:344 → 431

C2; C2 domain
XM_006498054.4 → XP_006498117.1 serine/threonine-protein kinase N3 isoform X7

See identical proteins and their annotated locations for XP_006498117.1

UniProtKB/TrEMBL

Q3UQS3

Related

ENSMUST00000137240.8

Conserved Domains (3) summary

cd05589
Location:426 → 751

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:41 → 99

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:199 → 286

C2; C2 domain