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    Nit1 nitrilase 1 [ Mus musculus (house mouse) ]

    Gene ID: 27045, updated on 25-Apr-2024

    Summary

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    Official Symbol
    Nit1provided by MGI
    Official Full Name
    nitrilase 1provided by MGI
    Primary source
    MGI:MGI:1350916
    See related
    Ensembl:ENSMUSG00000013997 AllianceGenome:MGI:1350916
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    ESTM30
    Summary
    Enables deaminated glutathione amidase activity. Involved in cellular amide catabolic process. Located in mitochondrion. Is expressed in several structures, including genitourinary system; liver; mammary gland; musculature; and spleen. Orthologous to human NIT1 (nitrilase 1). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in kidney adult (RPKM 60.8), testis adult (RPKM 53.4) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Nit1 in Genome Data Viewer
    Location:
    1 H3; 1 79.35 cM
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (171168145..171173209, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (171340571..171345656, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 25151 Neighboring gene predicted gene, 20045 Neighboring gene death effector domain-containing Neighboring gene STARR-positive B cell enhancer ABC_E2019 Neighboring gene prefoldin 2 Neighboring gene STARR-positive B cell enhancer ABC_E4387 Neighboring gene kelch domain containing 9 Neighboring gene nectin cell adhesion molecule 4

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Mammalian nitrilase 1 homologue Nit1 is a negative regulator in T cells. Zhang H, et al. Int Immunol, 2009 Jun. PMID 19395373, Free PMC Article
    2. Nit1 and Fhit tumor suppressor activities are additive. Sun J, et al. J Cell Biochem, 2009 Aug 15. PMID 19479888, Free PMC Article
    3. Nitrilase 1 modulates lung tumor progression in vitro and in vivo. Wang YA, et al. Oncotarget, 2016 Apr 19. PMID 26967383, Free PMC Article
    4. Effect of zinc supplementation on N-nitrosomethylbenzylamine-induced forestomach tumor development and progression in tumor suppressor-deficient mouse strains. Sun J, et al. Carcinogenesis, 2011 Mar. PMID 21097531, Free PMC Article
    5. Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes. Barglow KT, et al. Biochemistry, 2008 Dec 23. PMID 19053248, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo.
      Title: Nitrilase 1 modulates lung tumor progression in vitro and in vivo.
    2. the extent of tumor susceptibility due to Nit1 and Fhit deficiency is additive
      Title: Nit1 and Fhit tumor suppressor activities are additive.
    3. genetic deficiency results in T cell hyperproliferative responses induced by TCR stimulation, spontaneous cell cycle entry and enhanced cell cycle progression
      Title: Mammalian nitrilase 1 homologue Nit1 is a negative regulator in T cells.
    4. Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies.
      Title: Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables deaminated glutathione amidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in amide catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    deaminated glutathione amidase
    Names
    dGSH amidase
    nitrilase homolog 1
    NP_001229509.1
    NP_001407610.1
    NP_001407611.1
    NP_001407612.1
    NP_001407613.1
    NP_036179.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001242580.2 → NP_001229509.1  deaminated glutathione amidase isoform 2

      See identical proteins and their annotated locations for NP_001229509.1

      Status: VALIDATED

      Source sequence(s)
      AC087229
      Consensus CDS
      CCDS56656.1
      UniProtKB/Swiss-Prot
      Q8VDK1
      Related
      ENSMUSP00000106920.2, ENSMUST00000111289.8
      Conserved Domains (1) summary
      cd07572
      Location:11 → 278
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    2. NM_001420681.1 → NP_001407610.1  deaminated glutathione amidase isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC087229

    3. NM_001420682.1 → NP_001407611.1  deaminated glutathione amidase isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC087229

    4. NM_001420683.1 → NP_001407612.1  deaminated glutathione amidase isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC087229

    5. NM_001420684.1 → NP_001407613.1  deaminated glutathione amidase isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC087229

    6. NM_012049.3 → NP_036179.1  deaminated glutathione amidase isoform 1

      See identical proteins and their annotated locations for NP_036179.1

      Status: VALIDATED

      Source sequence(s)
      AC087229
      Consensus CDS
      CCDS35775.1
      UniProtKB/Swiss-Prot
      O88526, Q8VDK1, Q9R1N4
      Related
      ENSMUSP00000106926.2, ENSMUST00000111295.8
      Conserved Domains (1) summary
      cd07572
      Location:44 → 311
      nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      171168145..171173209 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8VDK1.2 GenPept UniProtKB/Swiss-Prot:Q8VDK1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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