Vamp8 vesicle-associated membrane protein 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Vamp8provided by MGI
Official Full Name: vesicle-associated membrane protein 8provided by MGI
Primary source: MGI:MGI:1336882
See related: Ensembl:ENSMUSG00000050732 AllianceGenome:MGI:1336882
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Edb; endobrevin
Summary: Predicted to enable SNAP receptor activity; chloride channel inhibitor activity; and syntaxin binding activity. Involved in SNARE complex assembly and mucus secretion. Acts upstream of or within several processes, including cellular response to interferon-gamma; positive regulation of pancreatic amylase secretion; and zymogen granule exocytosis. Located in several cellular components, including basolateral plasma membrane; cytoplasmic vesicle; and lysosome. Part of SNARE complex. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and immune system. Orthologous to human VAMP8 (vesicle associated membrane protein 8). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in kidney adult (RPKM 138.4), lung adult (RPKM 135.0) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 C1; 6 32.27 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (72362204..72367650, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (72385221..72390667, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Clearance of intracellular tau protein from neuronal cells via VAMP8-induced secretion.
Title: Clearance of intracellular tau protein from neuronal cells via VAMP8-induced secretion.
in addition to a secretory role, VAMP8-mediates trafficking of NOX2 to endosomes and phagosomes and this promotes induction of cytolytic T cell immune responses
Title: VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release.
This report highlights the importance of the VAMP8 secretory machinery in facilitating mucus release from intestinal goblet cells and the dire consequences that occur during disease pathogenesis if these pathways are not functional.
Title: Entamoeba histolytica-Induced Mucin Exocytosis Is Mediated by VAMP8 and Is Critical in Mucosal Innate Host Defense.
acute inhibition of VAMP8-mediated secretion during pancreatitis triggers intracellular trypsin accumulation and loss of the early endosomal compartment
Title: Acute acinar pancreatitis blocks vesicle-associated membrane protein 8 (VAMP8)-dependent secretion, resulting in intracellular trypsin accumulation.
These findings reveal a role for VAMP8 in LC3-associated phagocytosis and highlight a novel mechanism exploited by L. major promastigotes to interfere with the host antimicrobial machinery
Title: Leishmania major Promastigotes Evade LC3-Associated Phagocytosis through the Action of GP63.
VAMP8-mediated secretion is dependent on anterograde endosomal trafficking.
Title: Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Leishmania evades host immunity by GP63-mediated VAMP8 cleavage.
Title: Leishmania evades host immunity by inhibiting antigen cross-presentation through direct cleavage of the SNARE VAMP8.
VAMP8 mediates insulin granule recruitment to the plasma membrane, which partly accounts for GLP-1 potentiation of glucose-stimulated insulin secretion.
Title: Dual role of VAMP8 in regulating insulin exocytosis and islet β cell growth.
VAMP8 knockout mice were used to show that the SNARE machinery plays a critical role in the process of granule homotypic fusion.
Title: VAMP8/endobrevin is a critical factor for the homotypic granule growth in pancreatic acinar cells.
VAMP8 regulates mucin granule exocytosis in airway goblet cells, and reduction of its expression may provide a novel therapeutic target to ameliorate airway mucus obstruction in lung diseases
Title: VAMP8 is a vesicle SNARE that regulates mucin secretion in airway goblet cells.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (4) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH101850 (e-PCR)
- UniSTS:116759

AF053724 (e-PCR)
- UniSTS:163391

Vamp8 (e-PCR), detects polymorphism
- UniSTS:144743

AU041171 (e-PCR)
- UniSTS:179394

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SNAP receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNAP receptor activity	ISO Inferred from Sequence Orthology more info
enables chloride channel inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in SNARE complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in SNARE complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in autophagosome maturation	ISO Inferred from Sequence Orthology more info
involved_in autophagosome membrane docking	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within autophagy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to type II interferon	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within defense response to virus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane fusion	ISO Inferred from Sequence Orthology more info
involved_in mucus secretion	IBA Inferred from Biological aspect of Ancestor more info
involved_in mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mucus secretion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of secretion by cell	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of histamine secretion by mast cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of pancreatic amylase secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within symbiont entry into host cell	ISO Inferred from Sequence Orthology more info	PubMed
involved_in vesicle fusion	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within vesicle fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle fusion	ISO Inferred from Sequence Orthology more info
involved_in vesicle-mediated transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within zymogen granule exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of SNARE complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SNARE complex	IDA Inferred from Direct Assay more info	PubMed
part_of SNARE complex	ISO Inferred from Sequence Orthology more info	PubMed
located_in azurophil granule membrane	ISO Inferred from Sequence Orthology more info
located_in basal part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in early endosome	TAS Traceable Author Statement more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in late endosome membrane	ISO Inferred from Sequence Orthology more info
located_in lysosomal membrane	ISO Inferred from Sequence Orthology more info
located_in lysosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mucin granule	IBA Inferred from Biological aspect of Ancestor more info
located_in mucin granule	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in phagocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in recycling endosome	ISO Inferred from Sequence Orthology more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule membrane	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info
located_in zymogen granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: vesicle-associated membrane protein 8

Names: VAMP-8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.