Prss50 serine protease 50 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prss50provided by MGI
Official Full Name: serine protease 50provided by MGI
Primary source: MGI:MGI:2447303
See related: Ensembl:ENSMUSG00000048752 AllianceGenome:MGI:2447303
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tsp50; 79H19C
Summary: Predicted to enable threonine-type endopeptidase activity. Predicted to be involved in proteolysis. Predicted to be located in cytoplasm. Predicted to be active in endoplasmic reticulum. Is expressed in liver. Orthologous to human PRSS50 (serine protease 50). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 29.0), liver E14.5 (RPKM 5.0) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F2; 9 60.79 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (110687032..110693697)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (110857964..110864629)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TSP50 Attenuates DSS-Induced Colitis by Regulating TGF-beta Signaling Mediated Maintenance of Intestinal Mucosal Barrier Integrity.
Title: TSP50 Attenuates DSS-Induced Colitis by Regulating TGF-β Signaling Mediated Maintenance of Intestinal Mucosal Barrier Integrity.
PRSS50 is a testis protease responsible for proper sperm tail formation and function.
Title: PRSS50 is a testis protease responsible for proper sperm tail formation and function.
TSP50 expression at embryonic day 6.5 (E6.5), day 8.5 (E8.5) and day 10.5 (E10.5) was increasingly enhanced, However, the expression of TSP50 decreased gradually in the development and differentiation of cardiac myocyte from E12.5 to postnatal (P0).
Title: Expression and effects of TSP50 in mouse embryo and cardiac myocyte development.
findings indicate that BORIS-induced expression of TSP50 is governed by accessibility and binding of BORIS to the promoter
Title: Transcription factor BORIS (Brother of the Regulator of Imprinted Sites) directly induces expression of a cancer-testis antigen, TSP50, through regulated binding of BORIS to the promoter.
TSP50 promotes cell proliferation, at least partially, through activation of the NF-kappaB signalling pathway.
Title: Testes-specific protease 50 (TSP50) promotes cell proliferation through the activation of the nuclear factor κB (NF-κB) signalling pathway.
results showed that downregulation of TSP50 expression in P19 embryonal carcinoma stem cells not only reduced cell proliferation, colony formation, and migration but also induced cell apoptosis
Title: Knockdown of TSP50 inhibits cell proliferation and induces apoptosis in P19 cells.
These findings suggested that DNA methylation might regulate the TSP50 and mTSP50 gene expressions in different types of tissues and spermatic cells.
Title: Differential methylation of TSP50 and mTSP50 genes in different types of human tissues and mouse spermatic cells.
TSP50 may perform a critical biologic function in mammalian spermatogenesis. It may be a new type of threonine protease whose linear catalytic structure is similar to many serine proteases, except for a Thr-for-Ser residual catalytic site substitution.
Title: Localization and expression of TSP50 protein in human and rodent testes.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (1)
Endonuclease-mediated (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:488464

NoName (e-PCR)
- UniSTS:494065

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables threonine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables threonine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in proteolysis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: probable threonine protease PRSS50

Names: protease, serine 50; testes-specific protease 50; testis-specific protease-like protein 50

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001379367.1 → NP_001366296.1 probable threonine protease PRSS50 isoform 2

Status: VALIDATED

Source sequence(s)

AC139378

Conserved Domains (1) summary

cd00190
Location:151 → 381

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...
NM_146227.4 → NP_666339.2 probable threonine protease PRSS50 isoform 1 precursor

See identical proteins and their annotated locations for NP_666339.2

Status: VALIDATED

Source sequence(s)

AC139378

Consensus CDS

CCDS23577.1

UniProtKB/Swiss-Prot

B2RWS9, Q8BLH5, Q8K466

Related

ENSMUSP00000059668.2, ENSMUST00000051097.6

Conserved Domains (2) summary

smart00020
Location:177 → 407

Tryp_SPc; Trypsin-like serine protease

cd00190
Location:177 → 407

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...