Tnni3k TNNI3 interacting kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tnni3kprovided by MGI
Official Full Name: TNNI3 interacting kinaseprovided by MGI
Primary source: MGI:MGI:2443276
See related: Ensembl:ENSMUSG00000040086 AllianceGenome:MGI:2443276
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cark; D830019J24Rik
Summary: Predicted to enable protein C-terminus binding activity; protein serine/threonine kinase activity; and troponin I binding activity. Involved in regulation of cardiac conduction and regulation of heart rate. Predicted to be located in cytoplasm and nucleus. Orthologous to several human genes including FPGT-TNNI3K (FPGT-TNNI3K readthrough). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward heart adult (RPKM 27.2) See more
Orthologs: human all
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Genomic context

Location:: 3 H4; 3 79.32 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (154491928..154761044, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (154786291..155055407, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Tnni3k influences cardiomyocyte S-phase activity and proliferation.
Title: Tnni3k influences cardiomyocyte S-phase activity and proliferation.
An improved method reveals the dual role of Tnni3k in promoting S-phase entry while suppressing cell division in cardiomyocytes.
Title: An improved method reveals the dual role of Tnni3k in promoting S-phase entry while suppressing cell division in cardiomyocytes.
Expression Levels of the Tnni3k Gene in the Heart Are Highly Associated with Cardiac and Glucose Metabolism-Related Phenotypes and Functional Pathways.
Title: Expression Levels of the Tnni3k Gene in the Heart Are Highly Associated with Cardiac and Glucose Metabolism-Related Phenotypes and Functional Pathways.
Cardiac Troponin I-Interacting Kinase Affects Cardiomyocyte S-Phase Activity but Not Cardiomyocyte Proliferation.
Title: Cardiac Troponin I-Interacting Kinase Affects Cardiomyocyte S-Phase Activity but Not Cardiomyocyte Proliferation.
The prevalent I686T human variant and loss-of-function mutations in the cardiomyocyte-specific kinase gene TNNI3K cause adverse contractility and concentric remodeling in mice.
Title: The prevalent I686T human variant and loss-of-function mutations in the cardiomyocyte-specific kinase gene TNNI3K cause adverse contractility and concentric remodeling in mice.
This may be explained by the observation that Tnni3k function in mice converges with oxidative stress to regulate mononuclear diploid cardiomyocyte frequency.
Title: Tnni3k alleles influence ventricular mononuclear diploid cardiomyocyte frequency.
using novel genetic animal models and newly developed small-molecule TNNI3K inhibitors, we demonstrated that TNNI3K-mediated IR injury occurs through impaired mitochondrial function and is in part dependent on p38 MAPK.
Title: Troponin I-interacting protein kinase: a novel cardiac-specific kinase, emerging as a molecular target for the treatment of cardiac disease.
Our data provide the first evidence for a role of Tnni3k in controlling the electrocardiographic PR interval indicating a function of Tnni3k in atrio-ventricular conduction
Title: Dissection of a quantitative trait locus for PR interval duration identifies Tnni3k as a novel modulator of cardiac conduction.
Data identified Tnni3k, Fpgt and H28 as likely likely candidates for Vms1.
Title: Quantitative trait locus analysis, pathway analysis, and consomic mapping show genetic variants of Tnni3k, Fpgt, or H28 control susceptibility to viral myocarditis.
Tnni3k plays a critical role in the modulation of different forms of heart disease, and this protein may provide a novel target for therapeutic intervention.
Title: Tnni3k modifies disease progression in murine models of cardiomyopathy.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (2) 1 citation
Targeted (2) 1 citation
Endonuclease-mediated (8) 1 citation

General gene information

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Markers

D3Mit219 (e-PCR), detects polymorphism
- UniSTS:129639

D3Mit234 (e-PCR), detects polymorphism
- UniSTS:129654

D3Mit115 (e-PCR), detects polymorphism
- UniSTS:129520

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cardiac conduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of heart rate	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase TNNI3K

Names: cardiac ankyrin repeat kinase

NP_796040.3

EC 2.7.11.1

XP_011238456.1

EC 2.7.11.1

XP_011238457.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_177066.5 → NP_796040.3 serine/threonine-protein kinase TNNI3K

See identical proteins and their annotated locations for NP_796040.3

Status: VALIDATED

Source sequence(s)

AC124174, AC125097, AC144762, BY452372

Consensus CDS

CCDS17928.1

UniProtKB/Swiss-Prot

B2RTJ7, Q5GIG5, Q5GIG6

Related

ENSMUSP00000070561.4, ENSMUST00000064076.6

Conserved Domains (6) summary

smart00219
Location:462 → 718

TyrKc; Tyrosine kinase, catalytic domain

cd00204
Location:99 → 220

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd14064
Location:468 → 721

PKc_TNNI3K; Catalytic domain of the Dual-specificity protein kinase, TNNI3-interacting kinase

pfam12796
Location:138 → 231

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:339 → 400

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:134 → 164

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

154491928..154761044 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011240155.1 → XP_011238457.1 serine/threonine-protein kinase TNNI3K isoform X1

See identical proteins and their annotated locations for XP_011238457.1

Related

ENSMUSP00000122478.2, ENSMUST00000143410.7

Conserved Domains (4) summary

PTZ00322
Location:2 → 156

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

PHA03095
Location:113 → 413

PHA03095; ankyrin-like protein; Provisional

cd14064
Location:468 → 706

PKc_TNNI3K; Catalytic domain of the Dual-specificity protein kinase, TNNI3-interacting kinase

sd00045
Location:134 → 164

ANK; ANK repeat [structural motif]
XM_011240154.1 → XP_011238456.1 serine/threonine-protein kinase TNNI3K isoform X1

See identical proteins and their annotated locations for XP_011238456.1

Conserved Domains (4) summary

PTZ00322
Location:2 → 156

PTZ00322; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase; Provisional

PHA03095
Location:113 → 413

PHA03095; ankyrin-like protein; Provisional

cd14064
Location:468 → 706

PKc_TNNI3K; Catalytic domain of the Dual-specificity protein kinase, TNNI3-interacting kinase

sd00045
Location:134 → 164

ANK; ANK repeat [structural motif]

RNA

XR_375539.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001012364.1: Suppressed sequence

Description

NM_001012364.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.