Triobp TRIO and F-actin binding protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Triobpprovided by MGI
Official Full Name: TRIO and F-actin binding proteinprovided by MGI
Primary source: MGI:MGI:1349410
See related: Ensembl:ENSMUSG00000033088 AllianceGenome:MGI:1349410
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tara; EST478828; mKIAA1662
Summary: This gene encodes a protein that interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility, and cell growth. The encoded protein also associates with F-actin and stabilizes F-actin structures. Domains contained in this encoded protein are an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. Mutations in the human gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants have been described [provided by RefSeq, Sep 2012]
Expression: Ubiquitous expression in adrenal adult (RPKM 14.9), ovary adult (RPKM 12.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 E1; 15 37.7 cM

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (78831924..78890069)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (78947724..79005869)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Unbalanced bidirectional radial stiffness gradients within the organ of Corti promoted by TRIOBP.
Title: Unbalanced bidirectional radial stiffness gradients within the organ of Corti promoted by TRIOBP.
ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points.
Title: ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points.
TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing.
Title: TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing.
missense mutations in the Pejvakin (PJVK or DFNB59) gene were first identified in patients with audiological hallmarks of auditory neuropathy spectrum disorder, whereas all other PJVK alleles cause hearing loss of cochlear origin. These findings suggest that complex pathogenetic mechanisms underlie human deafness DFNB59.
Title: Pejvakin, a Candidate Stereociliary Rootlet Protein, Regulates Hair Cell Function in a Cell-Autonomous Manner.
R1 motif is the major actin-binding domain of TRIOBP-4, and the binding of R2 motif with actin filaments is nonspecific.
Title: R1 motif is the major actin-binding domain of TRIOBP-4.
Tara activates Rac1 through the Trio RhoGEF, which binds to E-cadherin and subsequently increases the phosphorylation of p38 and Tbx3, a transcriptional E-cadherin repressor.
Title: Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling.
mutant Triobp mice are profoundly deaf; stereocilia of Triobp mice develop normally but fail to form rootlets and are easier to deflect and damage.
Title: Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2)
Targeted (2) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within auditory receptor cell stereocilium organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of substrate adhesion-dependent cell spreading	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of substrate adhesion-dependent cell spreading	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in stereocilium base	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: TRIO and F-actin-binding protein

Names: protein Tara; trio-associated repeat on actin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001024716.1 → NP_001019887.1 TRIO and F-actin-binding protein isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) contains a distinct 5' UTR and 5' coding region, which causes translation initiation to occur at a downstream start codon, compared to variant 3. The encoded protein (isoform 1) has a shorter N-terminus when compared to isoform 3.

Source sequence(s)

AK085512, BX528408, BY199415, BY761337

Consensus CDS

CCDS37138.1

UniProtKB/TrEMBL

A0A2U3TZ82

Related

ENSMUSP00000105309.3, ENSMUST00000109687.9

Conserved Domains (3) summary

cd13275
Location:56 → 157

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain

pfam00169
Location:56 → 149

PH; PH domain

cl12013
Location:380 → 519

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
NM_001039155.1 → NP_001034244.1 TRIO and F-actin-binding protein isoform 4

See identical proteins and their annotated locations for NP_001034244.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) contains a distinct 3' UTR and lacks a portion of the 3' coding region, compared to variant 3. The resulting isoform (4) has a shorter C-terminus when compared to isoform 3.

Source sequence(s)

DQ228002

Consensus CDS

CCDS88802.1

UniProtKB/Swiss-Prot

Q99KW3

Related

ENSMUSP00000155397.2, ENSMUST00000140228.8

Conserved Domains (2) summary

PHA03247
Location:378 → 895

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:145 → 515

PHA03307; transcriptional regulator ICP4; Provisional
NM_001039156.1 → NP_001034245.1 TRIO and F-actin-binding protein isoform 3

See identical proteins and their annotated locations for NP_001034245.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) encodes the longest protein (isoform 3).

Source sequence(s)

BP768417, CX223548, DQ228000

Consensus CDS

CCDS37136.1

UniProtKB/Swiss-Prot

Q2PZW9, Q2Q402, Q2Q403, Q2Q404, Q6ZPK4, Q8C6T3, Q8CG90, Q99KW3

Related

ENSMUSP00000105312.4, ENSMUST00000109690.11

Conserved Domains (4) summary

PHA03247
Location:378 → 899

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:145 → 515

PHA03307; transcriptional regulator ICP4; Provisional

COG0419
Location:1667 → 2014

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd13275
Location:1443 → 1544

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
NM_138579.4 → NP_613045.3 TRIO and F-actin-binding protein isoform 5

See identical proteins and their annotated locations for NP_613045.3

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an in-frame exon, compared to variant 3, resulting in a shorter protein (isoform 5) when compared to isoform 3.

Source sequence(s)

BP768417, BU058377, CA322031, CA751843, CF531000, CF532339, CF533172, CN528900, CX223548, DQ278602

Consensus CDS

CCDS37137.1

UniProtKB/Swiss-Prot

Q99KW3

Related

ENSMUSP00000105311.3, ENSMUST00000109689.9

Conserved Domains (5) summary

cd13275
Location:1397 → 1498

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain

pfam00169
Location:1397 → 1490

PH; PH domain

pfam15665
Location:1726 → 1943

FAM184; Family with sequence similarity 184, A and B

cl12013
Location:1721 → 1860

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

cl23720
Location:1834 → 1950

RILP-like; Rab interacting lysosomal protein-like 1 and 2 (Rilpl1 and Rilpl2)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

78831924..78890069

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520284.1 → XP_006520347.1 TRIO and F-actin-binding protein isoform X1

See identical proteins and their annotated locations for XP_006520347.1

Conserved Domains (4) summary

PHA03247
Location:379 → 900

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:150 → 516

PHA03307; transcriptional regulator ICP4; Provisional

COG0419
Location:1668 → 2015

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd13275
Location:1444 → 1545

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_006520286.1 → XP_006520349.1 TRIO and F-actin-binding protein isoform X3

Conserved Domains (4) summary

PHA03247
Location:379 → 900

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:150 → 516

PHA03307; transcriptional regulator ICP4; Provisional

COG0419
Location:1622 → 1969

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd13275
Location:1398 → 1499

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_006520287.1 → XP_006520350.1 TRIO and F-actin-binding protein isoform X4

Conserved Domains (4) summary

PHA03247
Location:379 → 900

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:150 → 516

PHA03307; transcriptional regulator ICP4; Provisional

COG0419
Location:1596 → 1943

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd13275
Location:1372 → 1473

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_036159070.1 → XP_036014963.1 TRIO and F-actin-binding protein isoform X5

Conserved Domains (4) summary

PHA03247
Location:378 → 899

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:145 → 515

PHA03307; transcriptional regulator ICP4; Provisional

COG1196
Location:1583 → 1942

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd13275
Location:1371 → 1472

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_036159071.1 → XP_036014964.1 TRIO and F-actin-binding protein isoform X6

Conserved Domains (3) summary

PHA03247
Location:378 → 899

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:145 → 515

PHA03307; transcriptional regulator ICP4; Provisional

cd13275
Location:1397 → 1498

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_006520285.3 → XP_006520348.1 TRIO and F-actin-binding protein isoform X1

See identical proteins and their annotated locations for XP_006520348.1

Conserved Domains (4) summary

PHA03247
Location:379 → 900

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:150 → 516

PHA03307; transcriptional regulator ICP4; Provisional

COG0419
Location:1668 → 2015

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd13275
Location:1444 → 1545

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_017316365.3 → XP_017171854.1 TRIO and F-actin-binding protein isoform X2

UniProtKB/Swiss-Prot

Q2PZW9, Q2Q402, Q2Q403, Q2Q404, Q6ZPK4, Q8C6T3, Q8CG90, Q99KW3

Conserved Domains (4) summary

PHA03247
Location:378 → 899

PHA03247; large tegument protein UL36; Provisional

PHA03307
Location:145 → 515

PHA03307; transcriptional regulator ICP4; Provisional

COG0419
Location:1667 → 2014

SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]

cd13275
Location:1443 → 1544

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_006520289.2 → XP_006520352.1 TRIO and F-actin-binding protein isoform X7

Conserved Domains (2) summary

cd13275
Location:93 → 194

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain

TIGR02168
Location:366 → 664

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
XM_006520291.4 → XP_006520354.1 TRIO and F-actin-binding protein isoform X9

UniProtKB/TrEMBL

G5E8W1

Related

ENSMUSP00000122988.4, ENSMUST00000130663.10

Conserved Domains (1) summary

cd13275
Location:56 → 157

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain
XM_036159072.1 → XP_036014965.1 TRIO and F-actin-binding protein isoform X8

Conserved Domains (2) summary

cd13275
Location:27 → 128

PH_M-RIP; Myosin phosphatase-RhoA Interacting Protein Pleckstrin homology (PH) domain

TIGR02168
Location:300 → 598

SMC_prok_B; chromosome segregation protein SMC, common bacterial type