Cdk16 cyclin dependent kinase 16 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cdk16provided by MGI
Official Full Name: cyclin dependent kinase 16provided by MGI
Primary source: MGI:MGI:97516
See related: Ensembl:ENSMUSG00000031065 AllianceGenome:MGI:97516
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Crk5; Pctk1; Pctaire1
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including regulation of insulin secretion involved in cellular response to glucose stimulus; secretion by cell; and spermatogenesis. Colocalizes with synaptic vesicle. Is expressed in several structures, including alimentary system; genitourinary system; immune system; liver; and nervous system. Orthologous to human CDK16 (cyclin dependent kinase 16). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in cerebellum adult (RPKM 104.3), adrenal adult (RPKM 90.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: X A1.3; X 16.18 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (20554210..20566119)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (20687917..20699880)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CCNYL1 regulates spermatogenesis through the interaction and modulation of CDK16.
Title: CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse.
Pctaire1 exerts promyogenic effects by regulating myoblast migration and process formation during skeletal myogenesis
Title: Pctaire1/Cdk16 promotes skeletal myogenesis by inducing myoblast migration and fusion.
a novel function of BRSK2 in the regulation of GSIS in beta-cells via a PCTAIRE1-dependent mechanism and suggest that BRSK2 is an attractive target for developing novel diabetic drugs.
Title: Brain-selective kinase 2 (BRSK2) phosphorylation on PCTAIRE1 negatively regulates glucose-stimulated insulin secretion in pancreatic β-cells.
Mice lacking CDK16 developed normally, but male mice were infertile. Spermatozoa isolated from their epididymis displayed thinning and elongation of the annulus region, adopted a bent shape, and showed impaired motility.
Title: Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis.
Cdk5-dependent phosphorylation of Pctaire1 at Ser95 plays an important role in dendrite development
Title: Cyclin-dependent kinase 5-dependent phosphorylation of Pctaire1 regulates dendrite development.
findings demonstrate that Pctaire1 interacts with p35, both in vitro and in vivo, and that phosphorylation of Pctaire1 by Cdk5 enhances its kinase activity
Title: Pctaire1 interacts with p35 and is a novel substrate for Cdk5/p35.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pctk1 (e-PCR), detects polymorphism
- UniSTS:144145

AI327059 (e-PCR)
- UniSTS:209509

Pctk1 (e-PCR), detects polymorphism
- UniSTS:527478

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cyclin-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in growth hormone secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of insulin secretion involved in cellular response to glucose stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of insulin secretion involved in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of cyclin-dependent protein kinase holoenzyme complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in neuron projection	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info
colocalizes_with synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cyclin-dependent kinase 16

Names: PCTAIRE-motif protein kinase 1; cell division protein kinase 16; serine/threonine-protein kinase PCTAIRE-1

NP_001297385.1

EC 2.7.11.22

NP_001398634.1

EC 2.7.11.22

NP_001398636.1

EC 2.7.11.22

NP_035179.1

EC 2.7.11.22

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001310456.2 → NP_001297385.1 cyclin-dependent kinase 16 isoform 2

See identical proteins and their annotated locations for NP_001297385.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. Variants 2 and 4 encode the same protein (isoform 2), which has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AL807240

Consensus CDS

CCDS81103.1

UniProtKB/TrEMBL

Q3TM24, Q3UPI3, Q91WP4

Related

ENSMUSP00000111021.2, ENSMUST00000115364.8

Conserved Domains (1) summary

cd07873
Location:127 → 423

STKc_PCTAIRE1; Catalytic domain of the Serine/Threonine Kinase, PCTAIRE-1 kinase
NM_001411705.1 → NP_001398634.1 cyclin-dependent kinase 16 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (1).

Source sequence(s)

AL807240

UniProtKB/Swiss-Prot

Q04735

UniProtKB/TrEMBL

Q543G3
NM_001411707.1 → NP_001398636.1 cyclin-dependent kinase 16 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. Variants 2 and 4 encode the same isoform (2), which has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AL807240

UniProtKB/TrEMBL

Q3TM24
NM_011049.6 → NP_035179.1 cyclin-dependent kinase 16 isoform 1

See identical proteins and their annotated locations for NP_035179.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer protein (isoform 1). Variants 1 and 3 encode the same isoform (1).

Source sequence(s)

AL807240

Consensus CDS

CCDS30045.1

UniProtKB/Swiss-Prot

Q04735

UniProtKB/TrEMBL

Q3UPI3, Q543G3, Q91WP4

Related

ENSMUSP00000033380.7, ENSMUST00000033380.7

Conserved Domains (1) summary

cd07873
Location:162 → 458

STKc_PCTAIRE1; Catalytic domain of the Serine/Threonine Kinase, PCTAIRE-1 kinase