Ern2 endoplasmic reticulum to nucleus signalling 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ern2provided by MGI
Official Full Name: endoplasmic reticulum to nucleus signalling 2provided by MGI
Primary source: MGI:MGI:1349436
See related: Ensembl:ENSMUSG00000030866 AllianceGenome:MGI:1349436
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ern1; Ire1; Ire1b; mIre1; ire1-beta
Summary: Predicted to enable several functions, including ATP binding activity; magnesium ion binding activity; and unfolded protein binding activity. Involved in several processes, including positive regulation of JUN kinase activity; regulation of transcription, DNA-templated; and response to endoplasmic reticulum stress. Acts upstream of or within intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress. Located in endoplasmic reticulum quality control compartment. Part of IRE1-TRAF2-ASK1 complex. Is expressed in central nervous system and retina. Orthologous to human ERN2 (endoplasmic reticulum to nucleus signaling 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in colon adult (RPKM 71.3), stomach adult (RPKM 48.6) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F2; 7 65.53 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (121769116..121785454, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (122169893..122186239, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endoplasmic reticulum chaperone GRP78 participates in fluoride-induced autophagy in LS8 cells by regulating the IRE1-TRAF2-JNK pathway.
Title: Endoplasmic reticulum chaperone GRP78 participates in fluoride-induced autophagy in LS8 cells by regulating the IRE1-TRAF2-JNK pathway.
Polystyrene nanoplastics aggravates lipopolysaccharide-induced apoptosis in mouse kidney cells by regulating IRE1/XBP1 endoplasmic reticulum stress pathway via oxidative stress.
Title: Polystyrene nanoplastics aggravates lipopolysaccharide-induced apoptosis in mouse kidney cells by regulating IRE1/XBP1 endoplasmic reticulum stress pathway via oxidative stress.
Disruption of proteostasis causes IRE1 mediated reprogramming of alveolar epithelial cells.
Title: Disruption of proteostasis causes IRE1 mediated reprogramming of alveolar epithelial cells.
The epithelial-specific ER stress sensor ERN2/IRE1beta enables host-microbiota crosstalk to affect colon goblet cell development.
Title: The epithelial-specific ER stress sensor ERN2/IRE1β enables host-microbiota crosstalk to affect colon goblet cell development.
The IRE1/JNK signaling pathway regulates inflammation cytokines and production of glomerular extracellular matrix in the acute kidney injury to chronic kidney disease transition.
Title: The IRE1/JNK signaling pathway regulates inflammation cytokines and production of glomerular extracellular matrix in the acute kidney injury to chronic kidney disease transition.
Pharmacologic IRE1/XBP1s activation promotes systemic adaptive remodeling in obesity.
Title: Pharmacologic IRE1/XBP1s activation promotes systemic adaptive remodeling in obesity.
Reactive oxygen species-mediated endoplasmic reticulum stress response induces apoptosis of Mycobacterium avium-infected macrophages by activating regulated IRE1-dependent decay pathway.
Title: Reactive oxygen species-mediated endoplasmic reticulum stress response induces apoptosis of Mycobacterium avium-infected macrophages by activating regulated IRE1-dependent decay pathway.
Results suggest that inhibition of the IRE1 RNase activity and XBP1 markedly represses differentiation. In particular, IRE1 RNase activity is essential from the myoblast to early differentiation stage.
Title: IRE1-XBP1 Pathway of the Unfolded Protein Response Is Required during Early Differentiation of C2C12 Myoblasts.
Degradation of Blos1 mRNA by IRE1 promotes late endosome-mediated microautophagy of protein aggregates and protects cells from their cytotoxic effects.
Title: Degradation of Blos1 mRNA by IRE1 repositions lysosomes and protects cells from stress.
A positive feedback bistable ERN2-XBP1S pathway regulates MUC5B-dominated mucus obstruction in idiopathic pulmonary fibrosis (IPF), providing an unfolded protein response-dependent mechanism linking the MUC5B promoter rs35705950 polymorphism with IPF pathogenesis.
Title: XBP1S Regulates MUC5B in a Promoter Variant-Dependent Pathway in Idiopathic Pulmonary Fibrosis Airway Epithelia.

Submit: New GeneRIF Correction See all GeneRIFs (35)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (3)
Targeted (1) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA nuclease activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in IRE1-mediated unfolded protein response	IBA Inferred from Biological aspect of Ancestor more info
involved_in apoptotic chromosome condensation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in endoplasmic reticulum unfolded protein response	IEA Inferred from Electronic Annotation more info
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of JUN kinase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in rRNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in response to endoplasmic reticulum stress	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to unfolded protein	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of IRE1-TRAF2-ASK1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of IRE1-TRAF2-ASK1 complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum quality control compartment	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase/endoribonuclease IRE2

Names: endoplasmic reticulum (ER) to nucleus signalling 2; endoplasmic reticulum-to-nucleus signaling 2; inositol-requiring 1 (Yeast homologue); inositol-requiring 1 beta; inositol-requiring protein 2

NP_001303618.1

EC 2.7.11.1

NP_036146.2

EC 2.7.11.1

XP_011240119.1

EC 2.7.11.1

XP_011240120.1

EC 2.7.11.1

XP_011240121.1

EC 2.7.11.1

XP_011240122.1

EC 2.7.11.1

XP_011240124.1

EC 2.7.11.1

XP_011240125.1

EC 2.7.11.1

XP_017177771.1

EC 2.7.11.1

XP_030098474.1

EC 2.7.11.1

XP_030098475.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001316689.1 → NP_001303618.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform 2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AK139291, BC108999, BY098554

UniProtKB/TrEMBL

Q32MS5, Q3UTN5

Conserved Domains (5) summary

cd09769
Location:37 → 365

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cd10422
Location:771 → 896

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

smart00220
Location:507 → 767

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1520
Location:40 → 236

PQQ; Outer membrane protein assembly factor BamB, contains PQQ-like beta-propeller repeat [Cell wall/membrane/envelope biogenesis]

cd13982
Location:504 → 768

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1
NM_012016.3 → NP_036146.2 serine/threonine-protein kinase/endoribonuclease IRE2 isoform 1 precursor

See identical proteins and their annotated locations for NP_036146.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).

Source sequence(s)

AK139291, AK153659, BY098554

Consensus CDS

CCDS21813.1

UniProtKB/Swiss-Prot

Q3U5E3, Q9Z2E3

UniProtKB/TrEMBL

Q3UTN5

Related

ENSMUSP00000033153.8, ENSMUST00000033153.9

Conserved Domains (5) summary

cd09769
Location:37 → 365

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cd10422
Location:772 → 897

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

smart00220
Location:508 → 768

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1520
Location:40 → 236

PQQ; Outer membrane protein assembly factor BamB, contains PQQ-like beta-propeller repeat [Cell wall/membrane/envelope biogenesis]

cd13982
Location:505 → 769

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1

RNA

NR_133565.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses two alternate splice sites in internal exons, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC122232, AK139291, BY098554

Related

ENSMUST00000206198.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

121769116..121785454 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011241819.4 → XP_011240121.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X3

UniProtKB/TrEMBL

Q3UTN5

Conserved Domains (3) summary

cd09769
Location:37 → 365

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cd10422
Location:771 → 904

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:504 → 768

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1
XM_011241818.4 → XP_011240120.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X2

UniProtKB/TrEMBL

Q3UTN5

Conserved Domains (3) summary

cd09769
Location:37 → 365

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cd10422
Location:771 → 904

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:504 → 768

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1
XM_011241817.4 → XP_011240119.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X1

UniProtKB/TrEMBL

Q3UTN5

Conserved Domains (3) summary

cd09769
Location:37 → 365

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cd10422
Location:772 → 905

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:505 → 769

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1
XM_011241820.4 → XP_011240122.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X4

UniProtKB/TrEMBL

Q3UTN5

Conserved Domains (3) summary

cd10422
Location:751 → 884

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:484 → 748

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1

cl14874
Location:37 → 344

Luminal_IRE1_like; The Luminal domain, a dimerization domain, of Inositol-requiring protein 1-like proteins
XM_030242614.2 → XP_030098474.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X5

Conserved Domains (3) summary

cd10422
Location:588 → 721

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:321 → 585

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1

cl14874
Location:5 → 181

Luminal_IRE1_like; The Luminal domain, a dimerization domain, of Inositol-requiring protein 1-like proteins
XM_030242615.2 → XP_030098475.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X9

Conserved Domains (2) summary

cd10422
Location:513 → 646

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:246 → 510

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1
XM_011241823.4 → XP_011240125.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X8

Conserved Domains (2) summary

cd10422
Location:555 → 688

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:288 → 552

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1
XM_011241822.4 → XP_011240124.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X7

Conserved Domains (3) summary

cd10422
Location:576 → 709

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:309 → 573

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1

cl14874
Location:1 → 169

Luminal_IRE1_like; The Luminal domain, a dimerization domain, of Inositol-requiring protein 1-like proteins
XM_017322282.3 → XP_017177771.1 serine/threonine-protein kinase/endoribonuclease IRE2 isoform X6

UniProtKB/TrEMBL

Q3UTN5

Conserved Domains (2) summary

cd09769
Location:37 → 365

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cl21453
Location:505 → 680

PKc_like; Protein Kinases, catalytic domain