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    Cyb5r3 cytochrome b5 reductase 3 [ Mus musculus (house mouse) ]

    Gene ID: 109754, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Cyb5r3provided by MGI
    Official Full Name
    cytochrome b5 reductase 3provided by MGI
    Primary source
    MGI:MGI:94893
    See related
    Ensembl:ENSMUSG00000018042 AllianceGenome:MGI:94893
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    B5R; Dia1; Dia-1; WU:Cyb5r3; 0610016L08Rik; 2500002N19Rik
    Summary
    Predicted to enable cytochrome-b5 reductase activity, acting on NAD(P)H and nucleotide binding activity. Predicted to contribute to nitrite reductase (NO-forming) activity. Predicted to be involved in nitric oxide biosynthetic process. Predicted to act upstream of or within cholesterol biosynthetic process. Located in mitochondrial inner membrane. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and hemolymphoid system gland. Human ortholog(s) of this gene implicated in breast cancer and methemoglobinemia. Orthologous to human CYB5R3 (cytochrome b5 reductase 3). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in duodenum adult (RPKM 409.4), adrenal adult (RPKM 270.7) and 23 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    15 E1; 15 39.4 cM
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (83037696..83060641, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (83153495..83176440, complement)

    Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene polymerase (DNA-directed), delta interacting protein 3 Neighboring gene RNA U12, small nuclear Neighboring gene STARR-positive B cell enhancer ABC_E4142 Neighboring gene STARR-seq mESC enhancer starr_39316 Neighboring gene STARR-seq mESC enhancer starr_39317 Neighboring gene alpha 1,4-galactosyltransferase Neighboring gene STARR-seq mESC enhancer starr_39321 Neighboring gene STARR-positive B cell enhancer ABC_E5503 Neighboring gene predicted gene, 32886

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling. Bueno M, et al. JCI Insight, 2023 Mar 8. PMID 36749633, Free PMC Article
    2. Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress. Durgin BG, et al. J Mol Cell Cardiol, 2022 Jan. PMID 34536439, Free PMC Article
    3. Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice. Rodríguez-López S, et al. Geroscience, 2020 Jun. PMID 32323139, Free PMC Article
    4. Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation. Durgin BG, et al. JCI Insight, 2019 Oct 3. PMID 31487266, Free PMC Article
    5. Crystal structure of a complex between amino and carboxy terminal fragments of mDia1: insights into autoinhibition of diaphanous-related formins. Nezami A, et al. PLoS One, 2010 Sep 30. PMID 20927338, Free PMC Article

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cyb5r3 activation rescues secondary failure to sulfonylurea but not beta-cell dedifferentiation.
      Title: Cyb5r3 activation rescues secondary failure to sulfonylurea but not β-cell dedifferentiation.
    2. CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1alpha-JNK pathways.
      Title: CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1α-JNK pathways.
    3. Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3.
      Title: Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b(5) reductase-3.
    4. Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
      Title: Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
    5. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
      Title: The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
    6. Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
      Title: Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
    7. Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation.
      Title: Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation.
    8. Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
      Title: Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
    9. GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.
      Title: GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.
    10. Cyb5r3 links FoxO1-dependent mitochondrial dysfunction with beta-cell failure.
      Title: Cyb5r3 links FoxO1-dependent mitochondrial dysfunction with β-cell failure.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (3)  1 citation
    • Endonuclease-mediated (2) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ADP binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables AMP binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables FAD binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables FAD binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables NAD binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cytochrome-b5 reductase activity, acting on NAD(P)H ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables flavin adenine dinucleotide binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    contributes_to nitrite reductase (NO-forming) activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cholesterol biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cholesterol metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nitric oxide biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within steroid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within steroid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within sterol biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in endoplasmic reticulum membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in lipid droplet ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrial inner membrane HDA PubMed 
    located_in mitochondrial membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial outer membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of nitric-oxide synthase complex ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    NADH-cytochrome b5 reductase 3
    Names
    diaphorase 1 (NADH)
    NP_084063.1
    XP_006520345.2
    XP_006520346.1
    XP_030104133.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_029787.3 → NP_084063.1  NADH-cytochrome b5 reductase 3

      See identical proteins and their annotated locations for NP_084063.1

      Status: VALIDATED

      Source sequence(s)
      AI839369, BC043074
      Consensus CDS
      CCDS27698.1
      UniProtKB/Swiss-Prot
      Q9DCN2
      Related
      ENSMUSP00000018186.10, ENSMUST00000018186.16
      Conserved Domains (1) summary
      PLN02252
      Location:14 → 301
      PLN02252; nitrate reductase [NADPH]

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000081.7 Reference GRCm39 C57BL/6J

      Range
      83037696..83060641 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030248273.1 → XP_030104133.1  NADH-cytochrome b5 reductase 3 isoform X2

      UniProtKB/Swiss-Prot
      Q9DCN2
      Conserved Domains (1) summary
      PLN02252
      Location:14 → 301
      PLN02252; nitrate reductase [NADPH]

    2. XM_006520282.3 → XP_006520345.2  NADH-cytochrome b5 reductase 3 isoform X1

      Conserved Domains (1) summary
      PLN02252
      Location:33 → 320
      PLN02252; nitrate reductase [NADPH]

    3. XM_006520283.5 → XP_006520346.1  NADH-cytochrome b5 reductase 3 isoform X3

      UniProtKB/Swiss-Prot
      Q9DCN2
      Related
      ENSMUSP00000124062.3, ENSMUST00000162834.3
      Conserved Domains (1) summary
      PLN02252
      Location:15 → 278
      PLN02252; nitrate reductase [NADPH]
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9DCN2.3 GenPept UniProtKB/Swiss-Prot:Q9DCN2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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